Inhibitory effects of thromboxane A                         2                          generation by ginsenoside Ro due to attenuation of cytosolic phospholipase A                         2                          phosphorylation and arachidonic acid release

Jung Hae Shin; Hyuk Woo Kwon; Man Hee Rhee; Hwa Jin Park

doi:10.1016/j.jgr.2017.12.007

Inhibitory effects of thromboxane A ₂ generation by ginsenoside Ro due to attenuation of cytosolic phospholipase A ₂ phosphorylation and arachidonic acid release

Jung Hae Shin
, Hyuk Woo Kwon
, Man Hee Rhee
, Hwa Jin Park

Department of Veterinary Medicine

Inje University
Far East University

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Background: Thromboxane A ₂ (TXA ₂ ) induces platelet aggregation and promotes thrombus formation. Although ginsenoside Ro (G-Ro) from Panax ginseng is known to exhibit a Ca ²⁺ -antagonistic antiplatelet effect, whether it inhibits Ca ²⁺ -dependent cytosolic phospholipase A ₂ (cPLA _2α ) activity to prevent the release of arachidonic acid (AA), a TXA ₂ precursor, is unknown. In this study, we attempted to identify the mechanism underlying G-Ro-mediated TXA ₂ inhibition. Methods: We investigated whether G-Ro attenuates TXA ₂ production and its associated molecules, such as cyclooxygenase-1 (COX-1), TXA ₂ synthase (TXAS), cPLA _2α , mitogen-activated protein kinases, and AA. To assay COX-1 and TXAS, we used microsomal fraction of platelets. Results: G-Ro reduced TXA ₂ production by inhibiting AA release. It acted by decreasing the phosphorylation of cPLA _2α , p38-mitogen-activated protein kinase, and c-Jun N-terminal kinase1, rather than by inhibiting COX-1 and TXAS in thrombin-activated human platelets. Conclusion: G-Ro inhibits AA release to attenuate TXA ₂ production, which may counteract TXA ₂ -associated thrombosis.

Original language	English
Pages (from-to)	236-241
Number of pages	6
Journal	Journal of Ginseng Research
Volume	43
Issue number	2
DOIs	https://doi.org/10.1016/j.jgr.2017.12.007
State	Published - Apr 2019

Keywords

Arachidonic acid
Cytosolic phospholipase A
Ginsenoside Ro
Mitogen-activated protein kinases
Thromboxane A

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10.1016/j.jgr.2017.12.007

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Shin, J. H., Kwon, H. W., Rhee, M. H., & Park, H. J. (2019). Inhibitory effects of thromboxane A ₂ generation by ginsenoside Ro due to attenuation of cytosolic phospholipase A ₂ phosphorylation and arachidonic acid release Journal of Ginseng Research, 43(2), 236-241. https://doi.org/10.1016/j.jgr.2017.12.007

Shin, Jung Hae ; Kwon, Hyuk Woo ; Rhee, Man Hee et al. / Inhibitory effects of thromboxane A ₂ generation by ginsenoside Ro due to attenuation of cytosolic phospholipase A ₂ phosphorylation and arachidonic acid release In: Journal of Ginseng Research. 2019 ; Vol. 43, No. 2. pp. 236-241.

@article{3a9c457345e3441b842f26f2b4a334b4,

title = " Inhibitory effects of thromboxane A 2 generation by ginsenoside Ro due to attenuation of cytosolic phospholipase A 2 phosphorylation and arachidonic acid release ",

abstract = " Background: Thromboxane A 2 (TXA 2 ) induces platelet aggregation and promotes thrombus formation. Although ginsenoside Ro (G-Ro) from Panax ginseng is known to exhibit a Ca 2+ -antagonistic antiplatelet effect, whether it inhibits Ca 2+ -dependent cytosolic phospholipase A 2 (cPLA 2α ) activity to prevent the release of arachidonic acid (AA), a TXA 2 precursor, is unknown. In this study, we attempted to identify the mechanism underlying G-Ro-mediated TXA 2 inhibition. Methods: We investigated whether G-Ro attenuates TXA 2 production and its associated molecules, such as cyclooxygenase-1 (COX-1), TXA 2 synthase (TXAS), cPLA 2α , mitogen-activated protein kinases, and AA. To assay COX-1 and TXAS, we used microsomal fraction of platelets. Results: G-Ro reduced TXA 2 production by inhibiting AA release. It acted by decreasing the phosphorylation of cPLA 2α , p38-mitogen-activated protein kinase, and c-Jun N-terminal kinase1, rather than by inhibiting COX-1 and TXAS in thrombin-activated human platelets. Conclusion: G-Ro inhibits AA release to attenuate TXA 2 production, which may counteract TXA 2 -associated thrombosis.",

keywords = "Arachidonic acid, Cytosolic phospholipase A, Ginsenoside Ro, Mitogen-activated protein kinases, Thromboxane A",

author = "Shin, \{Jung Hae\} and Kwon, \{Hyuk Woo\} and Rhee, \{Man Hee\} and Park, \{Hwa Jin\}",

note = "Publisher Copyright: {\textcopyright} 2018",

year = "2019",

month = apr,

doi = "10.1016/j.jgr.2017.12.007",

language = "English",

volume = "43",

pages = "236--241",

journal = "Journal of Ginseng Research",

issn = "1226-8453",

publisher = "Elsevier B.V.",

number = "2",

}

Shin, JH, Kwon, HW, Rhee, MH & Park, HJ 2019, ' Inhibitory effects of thromboxane A ₂ generation by ginsenoside Ro due to attenuation of cytosolic phospholipase A ₂ phosphorylation and arachidonic acid release ', Journal of Ginseng Research, vol. 43, no. 2, pp. 236-241. https://doi.org/10.1016/j.jgr.2017.12.007

Inhibitory effects of thromboxane A ₂ generation by ginsenoside Ro due to attenuation of cytosolic phospholipase A ₂ phosphorylation and arachidonic acid release . / Shin, Jung Hae; Kwon, Hyuk Woo; Rhee, Man Hee et al.
In: Journal of Ginseng Research, Vol. 43, No. 2, 04.2019, p. 236-241.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Inhibitory effects of thromboxane A 2 generation by ginsenoside Ro due to attenuation of cytosolic phospholipase A 2 phosphorylation and arachidonic acid release

AU - Shin, Jung Hae

AU - Kwon, Hyuk Woo

AU - Rhee, Man Hee

AU - Park, Hwa Jin

PY - 2019/4

Y1 - 2019/4

N2 - Background: Thromboxane A 2 (TXA 2 ) induces platelet aggregation and promotes thrombus formation. Although ginsenoside Ro (G-Ro) from Panax ginseng is known to exhibit a Ca 2+ -antagonistic antiplatelet effect, whether it inhibits Ca 2+ -dependent cytosolic phospholipase A 2 (cPLA 2α ) activity to prevent the release of arachidonic acid (AA), a TXA 2 precursor, is unknown. In this study, we attempted to identify the mechanism underlying G-Ro-mediated TXA 2 inhibition. Methods: We investigated whether G-Ro attenuates TXA 2 production and its associated molecules, such as cyclooxygenase-1 (COX-1), TXA 2 synthase (TXAS), cPLA 2α , mitogen-activated protein kinases, and AA. To assay COX-1 and TXAS, we used microsomal fraction of platelets. Results: G-Ro reduced TXA 2 production by inhibiting AA release. It acted by decreasing the phosphorylation of cPLA 2α , p38-mitogen-activated protein kinase, and c-Jun N-terminal kinase1, rather than by inhibiting COX-1 and TXAS in thrombin-activated human platelets. Conclusion: G-Ro inhibits AA release to attenuate TXA 2 production, which may counteract TXA 2 -associated thrombosis.

AB - Background: Thromboxane A 2 (TXA 2 ) induces platelet aggregation and promotes thrombus formation. Although ginsenoside Ro (G-Ro) from Panax ginseng is known to exhibit a Ca 2+ -antagonistic antiplatelet effect, whether it inhibits Ca 2+ -dependent cytosolic phospholipase A 2 (cPLA 2α ) activity to prevent the release of arachidonic acid (AA), a TXA 2 precursor, is unknown. In this study, we attempted to identify the mechanism underlying G-Ro-mediated TXA 2 inhibition. Methods: We investigated whether G-Ro attenuates TXA 2 production and its associated molecules, such as cyclooxygenase-1 (COX-1), TXA 2 synthase (TXAS), cPLA 2α , mitogen-activated protein kinases, and AA. To assay COX-1 and TXAS, we used microsomal fraction of platelets. Results: G-Ro reduced TXA 2 production by inhibiting AA release. It acted by decreasing the phosphorylation of cPLA 2α , p38-mitogen-activated protein kinase, and c-Jun N-terminal kinase1, rather than by inhibiting COX-1 and TXAS in thrombin-activated human platelets. Conclusion: G-Ro inhibits AA release to attenuate TXA 2 production, which may counteract TXA 2 -associated thrombosis.

KW - Arachidonic acid

KW - Cytosolic phospholipase A

KW - Ginsenoside Ro

KW - Mitogen-activated protein kinases

KW - Thromboxane A

UR - https://www.scopus.com/pages/publications/85044681514

U2 - 10.1016/j.jgr.2017.12.007

DO - 10.1016/j.jgr.2017.12.007

M3 - Article

AN - SCOPUS:85044681514

SN - 1226-8453

VL - 43

SP - 236

EP - 241

JO - Journal of Ginseng Research

JF - Journal of Ginseng Research

IS - 2

ER -

Shin JH, Kwon HW, Rhee MH, Park HJ. Inhibitory effects of thromboxane A ₂ generation by ginsenoside Ro due to attenuation of cytosolic phospholipase A ₂ phosphorylation and arachidonic acid release Journal of Ginseng Research. 2019 Apr;43(2):236-241. doi: 10.1016/j.jgr.2017.12.007

Inhibitory effects of thromboxane A ₂ generation by ginsenoside Ro due to attenuation of cytosolic phospholipase A ₂ phosphorylation and arachidonic acid release

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Keywords

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