Abstract
IGF-II is known to induce the growth of keratinocytes and the level was significantly elevated in the tissue fluid of psoriatic lesion. However, the role of IGF-II in psoriasis is not well deigned. Because an inflammatory cytokine, interleukin-6 (IL-6) is overexpressed in psoriatic lesions, we explored whether IGF-II has some role in psoriasis through induction of IL-6. Therefore, the expression of IL-6 was analyzed after treatment of IGF-II in primary cultured psoriatic cells and human keratinocyte cell line, HaCaT. We found that IGF-II induced the IL-6 mRNA expression significantly. To investigate the inducing mechanism of IL-6 by IGF-II, we examined the promoter activity of IL-6 and the DNA binding activity of NFκB, a strong regulator of IL-6. Interestingly, IL-6 promoter activity and the binding activity of NFκB were remarkably increased by IGF-II. Western blot data that IκB was reduced by IGF-II significantly suggest that NFκB activation by IGF-II may be mediated through the downregulation of IκB. Therefore, these results suggest a novel role of IGF-II in psoriasis possibly by inducing IL-6 through the activation of NFκB mediated by downregulation of IκB. (C) 2000 Academic Press.
Original language | English |
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Pages (from-to) | 312-317 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 278 |
Issue number | 2 |
DOIs | |
State | Published - 19 Nov 2000 |
Keywords
- Insulin-like growth factor-II
- Interleukin-6
- NFκB
- Psoriasis