Introgression blast resistance gene (Pita, Pik-s, Pib, Piz-t, and Pi54), and blight resistance gene (Xa4 dan Xa7) into transgenic plant 940302-2 golden rice through marker-assisted selection

This study to create superior rice varieties resistant to blast and blight disease and accumulate Pro-vitamin A. The study was conducted transgenic rice PAC using Nagdong varieties which were then improved by IR36 (PAC/IR36). Then introgression gene to increase the gene resistance to blast and blight disease by crossing the Transgenic Golden PAC/IR36 with IR64 selected parent rice as a blast resistance gene, after obtaining F1 seeds planted until the F3 derivative is then selected and characterized. Our results phenotypic confirmation results from 2 line resistant against to blast by the rate of 0-1 and 5 lines resistant against to blight by the rate of 0.21-2.62 cm lesion length. Genotypic confirmation using MAS (Marker Assisted Selection), from 11 line, one detected resistance gene to blast and blight (Pi-ta, Pik-s, Piz-t, Pib, Pi54, Xa4, and Xa7) and high β-carotene. The character of this line is consistent with the recipient's parent that contains a gene that plays a role in the β-carotene biosynthesis process and according to the desired character trait that is resistant to the biotype blast and blight in Indonesia.