TY - JOUR
T1 - Investigation of the morphological and cytogenetic variations of an intraspecific Asiatic lily hybrid using 5S and 18S rDNA probes
AU - Islam, Md Mazharul
AU - Yesmin, Reshma
AU - Jung, Min Jung
AU - Kim, Hong Yul
AU - Kim, Chang Kil
AU - Lim, Ki Byung
N1 - Publisher Copyright:
© 2020, Korean Society for Horticultural Science.
PY - 2020/4/1
Y1 - 2020/4/1
N2 - This study aimed to determine the morphological and cytogenetic differences in an intraspecific Asiatic F1Lilium hybrid. The results indicated that leaf color and shape, flower morphology including color, spot size on petal, and filament color in the F1 hybrid showed significant variations as compared to parents, while days to flowering were similar to those of the parents. Moreover, stem height, leaf number, and leaf width showed distinct variations. Interestingly, the intraspecific F1 progeny obtained from Asiatic lily parents showed higher pollen viability. In addition, FISH results revealed significant variation in the number of 18S rDNA and 5S rDNA loci identified in both parents and the F1 progeny. The female (2n = 4x = 48) parent had 12 loci of 18S rDNA, whereas the male (2n = 2x = 24) parent and the F1 (2n = 3x = 36) had 8 and 11 loci of 18S rDNA, respectively. Moreover, the F1 progeny had 9 loci of 5S rDNA compared with the 7 and 4 loci identified in the female and male parents, respectively. All 5S rDNA signals were observed on the long arm in both the female parent and the F1; however, one 5S rDNA signal was observed on the short arm in the male parent. Therefore, this study demonstrated that the distribution of ribosomal DNA was greatly different in the F1 progeny than that of parents.
AB - This study aimed to determine the morphological and cytogenetic differences in an intraspecific Asiatic F1Lilium hybrid. The results indicated that leaf color and shape, flower morphology including color, spot size on petal, and filament color in the F1 hybrid showed significant variations as compared to parents, while days to flowering were similar to those of the parents. Moreover, stem height, leaf number, and leaf width showed distinct variations. Interestingly, the intraspecific F1 progeny obtained from Asiatic lily parents showed higher pollen viability. In addition, FISH results revealed significant variation in the number of 18S rDNA and 5S rDNA loci identified in both parents and the F1 progeny. The female (2n = 4x = 48) parent had 12 loci of 18S rDNA, whereas the male (2n = 2x = 24) parent and the F1 (2n = 3x = 36) had 8 and 11 loci of 18S rDNA, respectively. Moreover, the F1 progeny had 9 loci of 5S rDNA compared with the 7 and 4 loci identified in the female and male parents, respectively. All 5S rDNA signals were observed on the long arm in both the female parent and the F1; however, one 5S rDNA signal was observed on the short arm in the male parent. Therefore, this study demonstrated that the distribution of ribosomal DNA was greatly different in the F1 progeny than that of parents.
KW - F progeny
KW - FISH
KW - Lilium
KW - Loci
KW - Morphology
UR - http://www.scopus.com/inward/record.url?scp=85083536660&partnerID=8YFLogxK
U2 - 10.1007/s13580-019-00216-7
DO - 10.1007/s13580-019-00216-7
M3 - Article
AN - SCOPUS:85083536660
SN - 2211-3452
VL - 61
SP - 339
EP - 346
JO - Horticulture Environment and Biotechnology
JF - Horticulture Environment and Biotechnology
IS - 2
ER -