TY - JOUR
T1 - Isocryptomerin, a novel membrane-active antifungal compound from Selaginella tamariscina
AU - Lee, Juneyoung
AU - Choi, Yunjung
AU - Woo, Eun Rhan
AU - Lee, Dong Gun
PY - 2009/2/13
Y1 - 2009/2/13
N2 - Isocryptomerin is a biflavonoid isolated from Selaginella tamariscina used in traditional medicine. In this study, we investigated novel antifungal properties of isocryptomerin. The results indicated that isocryptomerin exerted antifungal activity in an energy-independent manner without remarkable hemolytic effects. To understand mode of action(s) of isocryptomerin, we conducted experiments on Candida albicans, a noted human pathogenic fungal strain. Flow cytometric analysis with bis-(1,3-dibutylbarbituric acid) trimethine oxonol [DiBAC4(3)], a translational membrane potential dye, regeneration test with fungal protoplasts, and fluorescence analysis with 1,6-diphenyl-1,3,5-hexatriene (DPH), a probe for membrane studies by depolarization, indicated that isocryptomerin could depolarize fungal plasma membrane. In conclusion, the results suggested that the antifungal activities of isocryptomerin might be due to its membrane-disruption mechanism(s).
AB - Isocryptomerin is a biflavonoid isolated from Selaginella tamariscina used in traditional medicine. In this study, we investigated novel antifungal properties of isocryptomerin. The results indicated that isocryptomerin exerted antifungal activity in an energy-independent manner without remarkable hemolytic effects. To understand mode of action(s) of isocryptomerin, we conducted experiments on Candida albicans, a noted human pathogenic fungal strain. Flow cytometric analysis with bis-(1,3-dibutylbarbituric acid) trimethine oxonol [DiBAC4(3)], a translational membrane potential dye, regeneration test with fungal protoplasts, and fluorescence analysis with 1,6-diphenyl-1,3,5-hexatriene (DPH), a probe for membrane studies by depolarization, indicated that isocryptomerin could depolarize fungal plasma membrane. In conclusion, the results suggested that the antifungal activities of isocryptomerin might be due to its membrane-disruption mechanism(s).
KW - Antifungal activity
KW - Biflavonoid
KW - Isocryptomerin
KW - Membrane-disruption mechanism(s)
UR - http://www.scopus.com/inward/record.url?scp=58549098767&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2008.12.030
DO - 10.1016/j.bbrc.2008.12.030
M3 - Article
C2 - 19101515
AN - SCOPUS:58549098767
SN - 0006-291X
VL - 379
SP - 676
EP - 680
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -
