Isolating and characterizing molecular markers associated with seedling-stage cold tolerance in rice

K. M. Kim; I. K. Chung; T. S. Kwak; J. K. Sohn

doi:10.1142/9789812814319_0128

Isolating and characterizing molecular markers associated with seedling-stage cold tolerance in rice

K. M. Kim, I. K. Chung, T. S. Kwak, J. K. Sohn

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

The DNA marker associated with cold tolerance was cloned from random amplified polymorphic DNA (RAPD) fragments amplified with genomic DNA of a rice cultivar, Dular. The nucleotide sequence revealed that the putative open reading frame was 511 base pairs and contained 169 amino acid residues. It is 79% and 57% identical to the rice cDNA (C26347) found in the GeneBank Database at the nucleotide and amino acid sequence levels, respectively. Through RAPD analysis for the cold tolerance of 94 F₂ plants from a cross between Dular (indica, cold-sensitive cultivar) and Toyohatamochi (japonica, coldtolerant cultivar), OPT8511 was confirmed to have strong association with cold tolerance of rice. The clone OPT8₅₁₁ was amplified from polymerase chain reaction of genomic DNA in 21 rice cultivars by custom-made primer. This marker could be of use in marker-assisted selection for cold tolerance in rice.

Original language	English
Title of host publication	Advances In Rice Genetics (In 2 Parts)
Publisher	World Scientific Publishing Co.
Pages	336-337
Number of pages	2
ISBN (Electronic)	9789812814319
DOIs	https://doi.org/10.1142/9789812814319_0128
State	Published - 1 Jan 2008

Access to Document

10.1142/9789812814319_0128

Cite this

@inbook{9658f2328d4847108d18568c6aa807b4,

title = "Isolating and characterizing molecular markers associated with seedling-stage cold tolerance in rice",

abstract = "The DNA marker associated with cold tolerance was cloned from random amplified polymorphic DNA (RAPD) fragments amplified with genomic DNA of a rice cultivar, Dular. The nucleotide sequence revealed that the putative open reading frame was 511 base pairs and contained 169 amino acid residues. It is 79% and 57% identical to the rice cDNA (C26347) found in the GeneBank Database at the nucleotide and amino acid sequence levels, respectively. Through RAPD analysis for the cold tolerance of 94 F2 plants from a cross between Dular (indica, cold-sensitive cultivar) and Toyohatamochi (japonica, coldtolerant cultivar), OPT8511 was confirmed to have strong association with cold tolerance of rice. The clone OPT8511 was amplified from polymerase chain reaction of genomic DNA in 21 rice cultivars by custom-made primer. This marker could be of use in marker-assisted selection for cold tolerance in rice.",

author = "Kim, {K. M.} and Chung, {I. K.} and Kwak, {T. S.} and Sohn, {J. K.}",

note = "Publisher Copyright: {\textcopyright} International Rice Research Institute 2003.",

year = "2008",

month = jan,

day = "1",

doi = "10.1142/9789812814319_0128",

language = "English",

pages = "336--337",

booktitle = "Advances In Rice Genetics (In 2 Parts)",

publisher = "World Scientific Publishing Co.",

}

TY - CHAP

T1 - Isolating and characterizing molecular markers associated with seedling-stage cold tolerance in rice

AU - Kim, K. M.

AU - Chung, I. K.

AU - Kwak, T. S.

AU - Sohn, J. K.

PY - 2008/1/1

Y1 - 2008/1/1

N2 - The DNA marker associated with cold tolerance was cloned from random amplified polymorphic DNA (RAPD) fragments amplified with genomic DNA of a rice cultivar, Dular. The nucleotide sequence revealed that the putative open reading frame was 511 base pairs and contained 169 amino acid residues. It is 79% and 57% identical to the rice cDNA (C26347) found in the GeneBank Database at the nucleotide and amino acid sequence levels, respectively. Through RAPD analysis for the cold tolerance of 94 F2 plants from a cross between Dular (indica, cold-sensitive cultivar) and Toyohatamochi (japonica, coldtolerant cultivar), OPT8511 was confirmed to have strong association with cold tolerance of rice. The clone OPT8511 was amplified from polymerase chain reaction of genomic DNA in 21 rice cultivars by custom-made primer. This marker could be of use in marker-assisted selection for cold tolerance in rice.

AB - The DNA marker associated with cold tolerance was cloned from random amplified polymorphic DNA (RAPD) fragments amplified with genomic DNA of a rice cultivar, Dular. The nucleotide sequence revealed that the putative open reading frame was 511 base pairs and contained 169 amino acid residues. It is 79% and 57% identical to the rice cDNA (C26347) found in the GeneBank Database at the nucleotide and amino acid sequence levels, respectively. Through RAPD analysis for the cold tolerance of 94 F2 plants from a cross between Dular (indica, cold-sensitive cultivar) and Toyohatamochi (japonica, coldtolerant cultivar), OPT8511 was confirmed to have strong association with cold tolerance of rice. The clone OPT8511 was amplified from polymerase chain reaction of genomic DNA in 21 rice cultivars by custom-made primer. This marker could be of use in marker-assisted selection for cold tolerance in rice.

UR - http://www.scopus.com/inward/record.url?scp=85153643703&partnerID=8YFLogxK

U2 - 10.1142/9789812814319_0128

DO - 10.1142/9789812814319_0128

M3 - Chapter

AN - SCOPUS:85153643703

SP - 336

EP - 337

BT - Advances In Rice Genetics (In 2 Parts)

PB - World Scientific Publishing Co.

ER -

Isolating and characterizing molecular markers associated with seedling-stage cold tolerance in rice

Abstract

Access to Document

Other files and links

Fingerprint

Cite this