Isolation and characterization of a Myoviridae bacteriophage against Staphylococcus aureus isolated from dairy cows with mastitis

Jee Eun Han; Ji Hyung Kim; Sun Young Hwang; Casiano H. Choresca; Sang Phil Shin; Jin Woo Jun; Ji Young Chai; Yong Ho Park; Se Chang Park

doi:10.1016/j.rvsc.2013.06.001

Isolation and characterization of a Myoviridae bacteriophage against Staphylococcus aureus isolated from dairy cows with mastitis

Jee Eun Han, Ji Hyung Kim, Sun Young Hwang, Casiano H. Choresca, Sang Phil Shin, Jin Woo Jun, Ji Young Chai, Yong Ho Park, Se Chang Park

Department of Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

A lytic bacteriophage (phage), designated SAH-1, was isolated from sewage effluent near a dairy cow farm in Gwacheon, South Korea to search for biocontrol agents against Staphylococcus aureus infections. The SAH-1 was morphologically classified as Myoviridae and possessed an approximate 144. kb double-stranded genomic DNA. The phage showed broad host ranges within S. aureus strains including methicillin-resistant strains, and its latent period and burst size were approximately 20. min and 100. PFU/cell, respectively. Moreover, morphologic and genomic analysis of SAH-1 revealed that the phage was closely related to other Myoviridae phages infecting Staphylococcus species. The bacteriolytic activity of phage SAH-1 at a multiplicity of infection (MOI) 1 and 100 indicated its efficiency for reducing bacterial growth. Based on these results, phage SAH-1 could be considered a potential therapeutic or prophylactic candidate against S. aureus infections.

Original language	English
Pages (from-to)	758-763
Number of pages	6
Journal	Research in Veterinary Science
Volume	95
Issue number	2
DOIs	https://doi.org/10.1016/j.rvsc.2013.06.001
State	Published - Oct 2013

Keywords

Bacteriophage
Mastitis
Myoviridae
SAH-1
Staphylococcus aureus

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title = "Isolation and characterization of a Myoviridae bacteriophage against Staphylococcus aureus isolated from dairy cows with mastitis",

abstract = "A lytic bacteriophage (phage), designated SAH-1, was isolated from sewage effluent near a dairy cow farm in Gwacheon, South Korea to search for biocontrol agents against Staphylococcus aureus infections. The SAH-1 was morphologically classified as Myoviridae and possessed an approximate 144. kb double-stranded genomic DNA. The phage showed broad host ranges within S. aureus strains including methicillin-resistant strains, and its latent period and burst size were approximately 20. min and 100. PFU/cell, respectively. Moreover, morphologic and genomic analysis of SAH-1 revealed that the phage was closely related to other Myoviridae phages infecting Staphylococcus species. The bacteriolytic activity of phage SAH-1 at a multiplicity of infection (MOI) 1 and 100 indicated its efficiency for reducing bacterial growth. Based on these results, phage SAH-1 could be considered a potential therapeutic or prophylactic candidate against S. aureus infections.",

keywords = "Bacteriophage, Mastitis, Myoviridae, SAH-1, Staphylococcus aureus",

author = "Han, {Jee Eun} and Kim, {Ji Hyung} and Hwang, {Sun Young} and Choresca, {Casiano H.} and Shin, {Sang Phil} and Jun, {Jin Woo} and Chai, {Ji Young} and Park, {Yong Ho} and Park, {Se Chang}",

year = "2013",

month = oct,

doi = "10.1016/j.rvsc.2013.06.001",

language = "English",

volume = "95",

pages = "758--763",

journal = "Research in Veterinary Science",

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T1 - Isolation and characterization of a Myoviridae bacteriophage against Staphylococcus aureus isolated from dairy cows with mastitis

AU - Han, Jee Eun

AU - Kim, Ji Hyung

AU - Hwang, Sun Young

AU - Choresca, Casiano H.

AU - Shin, Sang Phil

AU - Jun, Jin Woo

AU - Chai, Ji Young

AU - Park, Yong Ho

AU - Park, Se Chang

PY - 2013/10

Y1 - 2013/10

N2 - A lytic bacteriophage (phage), designated SAH-1, was isolated from sewage effluent near a dairy cow farm in Gwacheon, South Korea to search for biocontrol agents against Staphylococcus aureus infections. The SAH-1 was morphologically classified as Myoviridae and possessed an approximate 144. kb double-stranded genomic DNA. The phage showed broad host ranges within S. aureus strains including methicillin-resistant strains, and its latent period and burst size were approximately 20. min and 100. PFU/cell, respectively. Moreover, morphologic and genomic analysis of SAH-1 revealed that the phage was closely related to other Myoviridae phages infecting Staphylococcus species. The bacteriolytic activity of phage SAH-1 at a multiplicity of infection (MOI) 1 and 100 indicated its efficiency for reducing bacterial growth. Based on these results, phage SAH-1 could be considered a potential therapeutic or prophylactic candidate against S. aureus infections.

AB - A lytic bacteriophage (phage), designated SAH-1, was isolated from sewage effluent near a dairy cow farm in Gwacheon, South Korea to search for biocontrol agents against Staphylococcus aureus infections. The SAH-1 was morphologically classified as Myoviridae and possessed an approximate 144. kb double-stranded genomic DNA. The phage showed broad host ranges within S. aureus strains including methicillin-resistant strains, and its latent period and burst size were approximately 20. min and 100. PFU/cell, respectively. Moreover, morphologic and genomic analysis of SAH-1 revealed that the phage was closely related to other Myoviridae phages infecting Staphylococcus species. The bacteriolytic activity of phage SAH-1 at a multiplicity of infection (MOI) 1 and 100 indicated its efficiency for reducing bacterial growth. Based on these results, phage SAH-1 could be considered a potential therapeutic or prophylactic candidate against S. aureus infections.

KW - Bacteriophage

KW - Mastitis

KW - Myoviridae

KW - SAH-1

KW - Staphylococcus aureus

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AN - SCOPUS:84881550552

SN - 0034-5288

VL - 95

SP - 758

EP - 763

JO - Research in Veterinary Science

JF - Research in Veterinary Science

IS - 2

ER -

Isolation and characterization of a Myoviridae bacteriophage against Staphylococcus aureus isolated from dairy cows with mastitis

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Keywords

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