TY - JOUR
T1 - Isolation and characterization of a novel Escherichia coli O157:H7-specific phage as a biocontrol agent
AU - Lee, Cheonghoon
AU - Choi, In Young
AU - Park, Do Hyeon
AU - Park, Mi Kyung
N1 - Publisher Copyright:
© 2020 Springer Nature Switzerland AG.
PY - 2020/2/6
Y1 - 2020/2/6
N2 - Purpose: Escherichia coli O157:H7 is one of the major foodborne pathogens of global public concern. Bacteriophages (phages) have emerged as a promising alternative to antibiotics for controlling pathogenic bacteria. Here, a lytic E. coli O157:H7-specific phage (KFS-EC) was isolated, identified, and characterized to evaluate its potential as a biocontrol agent for E. coli O157:H7. Methods: KFS-EC was isolated from slaughterhouse in Korea. Morphological analysis, genomic analysis and several physiological tests were performed to identify and characterize the KFS-EC. Results: A specificity test indicated KFS-EC was strictly specific to E. coli O157:H7 strains among 60 bacterial strains tested. Morphological and phylogenetic analyses confirmed that KFS-EC belongs to the Rb49virus genus, Tevenvirinae subfamily, and the Myoviridae family of phages. KFS-EC genome consists of 164,725 bp and a total of 270 coding sequence features, of which 114 open reading frames (ORFs) were identified as phage functional genes. KFS-EC does not contain genes encoding lysogenic property and pathogenicity, which ensure its safe application. KFS-EC was relatively stable (~1 log decrease) under stressed conditions such as temperatures (20 °C-50 °C), pHs (3-11), organic solvents (ethanol and chloroform), and biocides (0.1% citric acid, 1% citric acid, and 0.1% peracetic acid). KFS-EC was able to inhibit E. coli O157:H7 efficiently at a multiplicity of infection (MOI) of 0.01 for 8 h with greater inhibitory effect and durability and was stable at 4 °C and 22 °C over a 12-week storage period. Conclusions: Our results suggest that KFS-EC could be used as a biocontrol agent to E. coli O157:H7.
AB - Purpose: Escherichia coli O157:H7 is one of the major foodborne pathogens of global public concern. Bacteriophages (phages) have emerged as a promising alternative to antibiotics for controlling pathogenic bacteria. Here, a lytic E. coli O157:H7-specific phage (KFS-EC) was isolated, identified, and characterized to evaluate its potential as a biocontrol agent for E. coli O157:H7. Methods: KFS-EC was isolated from slaughterhouse in Korea. Morphological analysis, genomic analysis and several physiological tests were performed to identify and characterize the KFS-EC. Results: A specificity test indicated KFS-EC was strictly specific to E. coli O157:H7 strains among 60 bacterial strains tested. Morphological and phylogenetic analyses confirmed that KFS-EC belongs to the Rb49virus genus, Tevenvirinae subfamily, and the Myoviridae family of phages. KFS-EC genome consists of 164,725 bp and a total of 270 coding sequence features, of which 114 open reading frames (ORFs) were identified as phage functional genes. KFS-EC does not contain genes encoding lysogenic property and pathogenicity, which ensure its safe application. KFS-EC was relatively stable (~1 log decrease) under stressed conditions such as temperatures (20 °C-50 °C), pHs (3-11), organic solvents (ethanol and chloroform), and biocides (0.1% citric acid, 1% citric acid, and 0.1% peracetic acid). KFS-EC was able to inhibit E. coli O157:H7 efficiently at a multiplicity of infection (MOI) of 0.01 for 8 h with greater inhibitory effect and durability and was stable at 4 °C and 22 °C over a 12-week storage period. Conclusions: Our results suggest that KFS-EC could be used as a biocontrol agent to E. coli O157:H7.
KW - Bacteriophage
KW - Biocontrol agent
KW - Escherichia coli O157:H7
KW - Lytic activity
KW - Stability
UR - http://www.scopus.com/inward/record.url?scp=85085320603&partnerID=8YFLogxK
U2 - 10.1007/s40201-020-00452-5
DO - 10.1007/s40201-020-00452-5
M3 - Article
AN - SCOPUS:85085320603
SN - 2052-336X
VL - 18
SP - 189
EP - 199
JO - Journal of Environmental Health Science and Engineering
JF - Journal of Environmental Health Science and Engineering
IS - 1
ER -