Isolation and characterization of Pepper mottle virus infecting tomato in Korea

Mi Kyeong Kim; Hae Ryun Kwak; Jung Heon Han; Sug Ju Ko; Su Heon Lee; Jin Woo Park; Miranda Gilda Jonson; Kook Hyung Kim; Jeong Soo Kim; Hong Soo Choi; Byeong Jin Cha

doi:10.5423/PPJ.2008.24.2.152

Isolation and characterization of Pepper mottle virus infecting tomato in Korea

Mi Kyeong Kim, Hae Ryun Kwak, Jung Heon Han, Sug Ju Ko, Su Heon Lee, Jin Woo Park, Miranda Gilda Jonson, Kook Hyung Kim, Jeong Soo Kim, Hong Soo Choi, Byeong Jin Cha

Department of Plant Medicine

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A peculiar virus-like disease of tomato showing yellow mosaic and necrotic spots on leaves and necrosis on veins, petioles and stems was observed at the Tomato Experimental Station (TES), Buyeo, Chungcheongnam- do, Korea. The disease incidence at TES fields ranged from 21 to 35% infecting different tomato cultivars. For this reason, to identify the virus infecting tomato and to characterize the virus based on biology, serology, cytology and at molecular level. Here, leaf samples were randomly collected from different infected tomato cultivars at TES fields and greenhouses and tested by ELISA using Pepper mottle virus (PepMoV) and Tomato mosaic virus (ToMV) antisera. Infected saps were mechanically inoculated in different host plants to test for pathogenicity, symptomatology and host ranges. Infected tissues and ultrathin sections were examined by electron microscopy. Finally, putative coat protein and 3'-untranslated region (CP/3f-UTR) fragment was amplified and cloned for sequence determination and analyzed its genetic relationship to existing PepMoY and PVY sequences at the Genbank. Results showed 69% of the samples were positive with PepMoV, 13% with ToMV and 19% were doubly infected with PepMoV and ToMV. Symptoms greatly varied from different host plants inoculated with tomato leaf sap- infected with PepMoV alone and discussed in detailed in this paper. Electron microscopy from infected tissues showed filamentous particles of 720-750 nm in length, a typical morphology and size of PepMoV. In addition, cylindrical inclusion bodies, pinwheels, scrolls and laminates with masses of fibrillar inclusions were also found in ultrathin sections. Alignment of the sequences of the CP/3'-UTR revealed >96% sequence identity with PepMoY and only <61. % with PVV. Taken together, all these evidences presented clearly indicated that the causal agent infecting tomato at TES was PepMoV and we designated this PepMoV infecting tomato as Tom-sd2 strain in this study.

Original language	English
Pages (from-to)	152-158
Number of pages	7
Journal	Plant Pathology Journal
Volume	24
Issue number	2
DOIs	https://doi.org/10.5423/PPJ.2008.24.2.152
State	Published - 2008

Keywords

Inclusion bodies
Occurrence
PepMoV
Tomato

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10.5423/PPJ.2008.24.2.152

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title = "Isolation and characterization of Pepper mottle virus infecting tomato in Korea",

abstract = "A peculiar virus-like disease of tomato showing yellow mosaic and necrotic spots on leaves and necrosis on veins, petioles and stems was observed at the Tomato Experimental Station (TES), Buyeo, Chungcheongnam- do, Korea. The disease incidence at TES fields ranged from 21 to 35% infecting different tomato cultivars. For this reason, to identify the virus infecting tomato and to characterize the virus based on biology, serology, cytology and at molecular level. Here, leaf samples were randomly collected from different infected tomato cultivars at TES fields and greenhouses and tested by ELISA using Pepper mottle virus (PepMoV) and Tomato mosaic virus (ToMV) antisera. Infected saps were mechanically inoculated in different host plants to test for pathogenicity, symptomatology and host ranges. Infected tissues and ultrathin sections were examined by electron microscopy. Finally, putative coat protein and 3'-untranslated region (CP/3f-UTR) fragment was amplified and cloned for sequence determination and analyzed its genetic relationship to existing PepMoY and PVY sequences at the Genbank. Results showed 69% of the samples were positive with PepMoV, 13% with ToMV and 19% were doubly infected with PepMoV and ToMV. Symptoms greatly varied from different host plants inoculated with tomato leaf sap- infected with PepMoV alone and discussed in detailed in this paper. Electron microscopy from infected tissues showed filamentous particles of 720-750 nm in length, a typical morphology and size of PepMoV. In addition, cylindrical inclusion bodies, pinwheels, scrolls and laminates with masses of fibrillar inclusions were also found in ultrathin sections. Alignment of the sequences of the CP/3'-UTR revealed >96% sequence identity with PepMoY and only <61. % with PVV. Taken together, all these evidences presented clearly indicated that the causal agent infecting tomato at TES was PepMoV and we designated this PepMoV infecting tomato as Tom-sd2 strain in this study.",

keywords = "Inclusion bodies, Occurrence, PepMoV, Tomato",

author = "Kim, {Mi Kyeong} and Kwak, {Hae Ryun} and Han, {Jung Heon} and Ko, {Sug Ju} and Lee, {Su Heon} and Park, {Jin Woo} and Jonson, {Miranda Gilda} and Kim, {Kook Hyung} and Kim, {Jeong Soo} and Choi, {Hong Soo} and Cha, {Byeong Jin}",

year = "2008",

doi = "10.5423/PPJ.2008.24.2.152",

language = "English",

volume = "24",

pages = "152--158",

journal = "Plant Pathology Journal",

issn = "1598-2254",

publisher = "Korean Society of Plant Pathology",

number = "2",

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T1 - Isolation and characterization of Pepper mottle virus infecting tomato in Korea

AU - Kim, Mi Kyeong

AU - Kwak, Hae Ryun

AU - Han, Jung Heon

AU - Ko, Sug Ju

AU - Lee, Su Heon

AU - Park, Jin Woo

AU - Jonson, Miranda Gilda

AU - Kim, Kook Hyung

AU - Kim, Jeong Soo

AU - Choi, Hong Soo

AU - Cha, Byeong Jin

PY - 2008

Y1 - 2008

N2 - A peculiar virus-like disease of tomato showing yellow mosaic and necrotic spots on leaves and necrosis on veins, petioles and stems was observed at the Tomato Experimental Station (TES), Buyeo, Chungcheongnam- do, Korea. The disease incidence at TES fields ranged from 21 to 35% infecting different tomato cultivars. For this reason, to identify the virus infecting tomato and to characterize the virus based on biology, serology, cytology and at molecular level. Here, leaf samples were randomly collected from different infected tomato cultivars at TES fields and greenhouses and tested by ELISA using Pepper mottle virus (PepMoV) and Tomato mosaic virus (ToMV) antisera. Infected saps were mechanically inoculated in different host plants to test for pathogenicity, symptomatology and host ranges. Infected tissues and ultrathin sections were examined by electron microscopy. Finally, putative coat protein and 3'-untranslated region (CP/3f-UTR) fragment was amplified and cloned for sequence determination and analyzed its genetic relationship to existing PepMoY and PVY sequences at the Genbank. Results showed 69% of the samples were positive with PepMoV, 13% with ToMV and 19% were doubly infected with PepMoV and ToMV. Symptoms greatly varied from different host plants inoculated with tomato leaf sap- infected with PepMoV alone and discussed in detailed in this paper. Electron microscopy from infected tissues showed filamentous particles of 720-750 nm in length, a typical morphology and size of PepMoV. In addition, cylindrical inclusion bodies, pinwheels, scrolls and laminates with masses of fibrillar inclusions were also found in ultrathin sections. Alignment of the sequences of the CP/3'-UTR revealed >96% sequence identity with PepMoY and only <61. % with PVV. Taken together, all these evidences presented clearly indicated that the causal agent infecting tomato at TES was PepMoV and we designated this PepMoV infecting tomato as Tom-sd2 strain in this study.

AB - A peculiar virus-like disease of tomato showing yellow mosaic and necrotic spots on leaves and necrosis on veins, petioles and stems was observed at the Tomato Experimental Station (TES), Buyeo, Chungcheongnam- do, Korea. The disease incidence at TES fields ranged from 21 to 35% infecting different tomato cultivars. For this reason, to identify the virus infecting tomato and to characterize the virus based on biology, serology, cytology and at molecular level. Here, leaf samples were randomly collected from different infected tomato cultivars at TES fields and greenhouses and tested by ELISA using Pepper mottle virus (PepMoV) and Tomato mosaic virus (ToMV) antisera. Infected saps were mechanically inoculated in different host plants to test for pathogenicity, symptomatology and host ranges. Infected tissues and ultrathin sections were examined by electron microscopy. Finally, putative coat protein and 3'-untranslated region (CP/3f-UTR) fragment was amplified and cloned for sequence determination and analyzed its genetic relationship to existing PepMoY and PVY sequences at the Genbank. Results showed 69% of the samples were positive with PepMoV, 13% with ToMV and 19% were doubly infected with PepMoV and ToMV. Symptoms greatly varied from different host plants inoculated with tomato leaf sap- infected with PepMoV alone and discussed in detailed in this paper. Electron microscopy from infected tissues showed filamentous particles of 720-750 nm in length, a typical morphology and size of PepMoV. In addition, cylindrical inclusion bodies, pinwheels, scrolls and laminates with masses of fibrillar inclusions were also found in ultrathin sections. Alignment of the sequences of the CP/3'-UTR revealed >96% sequence identity with PepMoY and only <61. % with PVV. Taken together, all these evidences presented clearly indicated that the causal agent infecting tomato at TES was PepMoV and we designated this PepMoV infecting tomato as Tom-sd2 strain in this study.

KW - Inclusion bodies

KW - Occurrence

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ER -

Isolation and characterization of Pepper mottle virus infecting tomato in Korea

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Keywords

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