Isolation and characterization of psacotheasin, a novel knottin-type antimicrobial peptide, from Psacothea hilaris

Jae Sam Hwang, Juneyoung Lee, Bomi Hwang, Sung Hee Nam, Eun Young Yun, Seong Ryul Kim, Dong Gun Lee

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

We report the isolation and characterization of a novel knottin-type antimicrobial peptide from the yellow-spotted long-horned beetle Psacothea hilaris. A cDNA encoding a 56-mer knottin-type propeptide was identified and its predicted molecular mass and pI were 5.92 kDa and 8.28, respectively. A 34-mer mature peptide was also selected and named herein as psacotheasin. The antimicrobial activity of chemically synthesized psacotheasin against human bacterial pathogens was subsequently investigated. The results showed that psacotheasin exerted potent activities against both Gram-positive and Gram-negative bacterial strains. The present study suggests that psacotheasin can be applied to develop novel therapeutic agents.

Original languageEnglish
Pages (from-to)708-711
Number of pages4
JournalJournal of Microbiology and Biotechnology
Volume20
Issue number4
DOIs
StatePublished - Apr 2010

Keywords

  • Antimicrobial peptide
  • Knottin-type peptide
  • Psacothea hilaris
  • Psacotheasin

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