Isolation and identification of antioxidant compound from the Lythrum salicaria L. roots

The roots of Lythrum salicaria L. were extracted in 80% aqueous MeOH and the concentrated extract was fractionated with EtOAc, n-BuOH, and H₂O, successively. The repeated silicagel and octadecyl SiO₂ column chromatographies of the EtOAc fractions led to isolation of an antioxidant compound and two major compounds. From the results of spectral data and the chemical characteristics including nuclear magnetic resonance, MS, and IR, the structures of compounds were determind as myricetin-3-O-β-D-glucopyranoside (1), oleanolic acid (2), betulinic acid (3). This is the first reported isolation of compounds (1, 2) from L. salicaria. Compound 1 as well as EtOAc, n-BuOH, and H₂O solvent fractions were evaluated for 2,2-dipicryl-1-phenylhydrazyl radical scavenging activity.