Isolation and identification of flavonoids from the roots of Brassica rapa ssp

Rak Hun Jeong; Qian Wu; Jin Gyeong Cho; Dae Young Lee; Sabina Shrestha; Min Ho Lee; Kyung Tae Lee; Myung Sook Choi; Tae Sook Jeong; Eun Mi Ahn; Hae Gon Chung; Yeong Deok Rho; Nam In Baek

doi:10.3839/jabc.2013.005

Isolation and identification of flavonoids from the roots of Brassica rapa ssp

Rak Hun Jeong, Qian Wu, Jin Gyeong Cho, Dae Young Lee, Sabina Shrestha, Min Ho Lee, Kyung Tae Lee, Myung Sook Choi, Tae Sook Jeong, Eun Mi Ahn, Hae Gon Chung, Yeong Deok Rho, Nam In Baek

School of Life Science and Biotechnology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The roots of Brassica rapa ssp. were extracted with 95% aqueous ethanol and the concentrated extracts were partitioned using ethyl acetate (EtOAc), n-butyl alcohol and H₂O, successively. From the EtOAc fraction, five flavonoids were isolated through repeated silica gel and octadecyl silica gel (ODS) column chromatography (c.c.). Based on NMR, mass spectrometry (MS) and IR spectroscopic data, the chemical structures of the compounds were determined to be licochalcone A (1), 4,4'-dihydroxy-3'-methoxychalcone (2), liquirtigenin (3), liquiritin (4), and isoliquiritin (5). This is the first report of these compounds isolated from the root of this plant.

Original language	English
Pages (from-to)	23-27
Number of pages	5
Journal	Journal of Applied Biological Chemistry
Volume	56
Issue number	1
DOIs	https://doi.org/10.3839/jabc.2013.005
State	Published - 2013

Keywords

4,4'-dihydroxy-3'-methoxy-chalcone
Brassica rapa ssp
Isoliquiritin
Licochalcone A
Liquiritin
Liquirtigenin

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10.3839/jabc.2013.005

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title = "Isolation and identification of flavonoids from the roots of Brassica rapa ssp",

abstract = "The roots of Brassica rapa ssp. were extracted with 95% aqueous ethanol and the concentrated extracts were partitioned using ethyl acetate (EtOAc), n-butyl alcohol and H2O, successively. From the EtOAc fraction, five flavonoids were isolated through repeated silica gel and octadecyl silica gel (ODS) column chromatography (c.c.). Based on NMR, mass spectrometry (MS) and IR spectroscopic data, the chemical structures of the compounds were determined to be licochalcone A (1), 4,4'-dihydroxy-3'-methoxychalcone (2), liquirtigenin (3), liquiritin (4), and isoliquiritin (5). This is the first report of these compounds isolated from the root of this plant.",

keywords = "4,4'-dihydroxy-3'-methoxy-chalcone, Brassica rapa ssp, Isoliquiritin, Licochalcone A, Liquiritin, Liquirtigenin",

author = "Jeong, {Rak Hun} and Qian Wu and Cho, {Jin Gyeong} and Lee, {Dae Young} and Sabina Shrestha and Lee, {Min Ho} and Lee, {Kyung Tae} and Choi, {Myung Sook} and Jeong, {Tae Sook} and Ahn, {Eun Mi} and Chung, {Hae Gon} and Rho, {Yeong Deok} and Baek, {Nam In}",

year = "2013",

doi = "10.3839/jabc.2013.005",

language = "English",

volume = "56",

pages = "23--27",

journal = "Journal of Applied Biological Chemistry",

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publisher = "The Korean Society for Applied Biological Chemistry",

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TY - JOUR

T1 - Isolation and identification of flavonoids from the roots of Brassica rapa ssp

AU - Jeong, Rak Hun

AU - Wu, Qian

AU - Cho, Jin Gyeong

AU - Lee, Dae Young

AU - Shrestha, Sabina

AU - Lee, Min Ho

AU - Lee, Kyung Tae

AU - Choi, Myung Sook

AU - Jeong, Tae Sook

AU - Ahn, Eun Mi

AU - Chung, Hae Gon

AU - Rho, Yeong Deok

AU - Baek, Nam In

PY - 2013

Y1 - 2013

N2 - The roots of Brassica rapa ssp. were extracted with 95% aqueous ethanol and the concentrated extracts were partitioned using ethyl acetate (EtOAc), n-butyl alcohol and H2O, successively. From the EtOAc fraction, five flavonoids were isolated through repeated silica gel and octadecyl silica gel (ODS) column chromatography (c.c.). Based on NMR, mass spectrometry (MS) and IR spectroscopic data, the chemical structures of the compounds were determined to be licochalcone A (1), 4,4'-dihydroxy-3'-methoxychalcone (2), liquirtigenin (3), liquiritin (4), and isoliquiritin (5). This is the first report of these compounds isolated from the root of this plant.

AB - The roots of Brassica rapa ssp. were extracted with 95% aqueous ethanol and the concentrated extracts were partitioned using ethyl acetate (EtOAc), n-butyl alcohol and H2O, successively. From the EtOAc fraction, five flavonoids were isolated through repeated silica gel and octadecyl silica gel (ODS) column chromatography (c.c.). Based on NMR, mass spectrometry (MS) and IR spectroscopic data, the chemical structures of the compounds were determined to be licochalcone A (1), 4,4'-dihydroxy-3'-methoxychalcone (2), liquirtigenin (3), liquiritin (4), and isoliquiritin (5). This is the first report of these compounds isolated from the root of this plant.

KW - 4,4'-dihydroxy-3'-methoxy-chalcone

KW - Brassica rapa ssp

KW - Isoliquiritin

KW - Licochalcone A

KW - Liquiritin

KW - Liquirtigenin

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U2 - 10.3839/jabc.2013.005

DO - 10.3839/jabc.2013.005

M3 - Article

AN - SCOPUS:84922687160

SN - 1976-0442

VL - 56

SP - 23

EP - 27

JO - Journal of Applied Biological Chemistry

JF - Journal of Applied Biological Chemistry

IS - 1

ER -

Isolation and identification of flavonoids from the roots of Brassica rapa ssp

Abstract

Keywords

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