Isolation and Identification of Ice Nucleation Active Fusarium Strains from Rapid Apple Declined Trees in Korea

Diane Avalos-Ruiz, Leonid N. Ten, Chang Kil Kim, Seung Yeol Lee, Hee Young Jung

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

In biological particles such as Fusarium species, ice nucleation activity (INA) has been observed. Fusarium strains isolated from apple declined trees in Korea were identified with a multilocus sequence analysis using the tef1 and rpb1 genes. Droplet-freezing and tube-freezing assays were used to determine the INA of the strains, using Pseudomonas syringae pv. syringae KACC 21200 as a positive control and resulting in seven INA+ fungal strains that were identified as F. tricinctum (KNUF-21-F17, KNUF-21-F18, KNUF-21-F29, KNUF-21-F32, KNUF-21-F38, KNUF-21-F43, and KNUF-21-F44). The effect of Fusarium INA+ KNUF-21-F29 was compared to that of INA– strains on Chrysanthemum morifolium cv. Shinma explants. A higher callus formation and no-shoot formation were observed, suggesting that fungal INA could play a role in cold injuries and be a factor to consider in rapid apple decline. To the best of our knowledge, this is the first report of INA fungal strains isolated in Korea.

Original languageEnglish
Pages (from-to)403-409
Number of pages7
JournalPlant Pathology Journal
Volume38
Issue number4
DOIs
StatePublished - Aug 2022

Keywords

  • cold injury
  • Fusarium
  • ice nucleation activity

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