Isolation, identification, and characterization of a keratin-degrading bacterium chryseobacterium sp. P1-3

Sung Jun Hong; Gun Seok Park; Byung Kwon Jung; Abdur Rahim Khan; Yeong Jun Park; Chang Hyun Lee; Jae Ho Shin

doi:10.3839/jabc.2015.039

Isolation, identification, and characterization of a keratin-degrading bacterium chryseobacterium sp. P1-3

Sung Jun Hong, Gun Seok Park, Byung Kwon Jung, Abdur Rahim Khan, Yeong Jun Park, Chang Hyun Lee, Jae Ho Shin

School of Applied Bioscience

Kyungpook National University

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

In this study, a keratin-degrading bacterium was isolated from soil contaminated with feather waste. The isolated strain was identified as Chryseobacterium sp. P1-3 on the basis of the 16S rRNA gene sequence alignment. Chryseobacterium sp. P1-3 is currently used in various biotechnological applications (e.g., in the hydrolysis of poultry feathers). It hydrolyzed the feather meal within 2 days and possesses a high level of keratinase activity (98 U/mL). The keratinase, partially purified from this strain, prefers casein as a substrate and shows optimal activity at a temperature of 30oC and at a pH of 8.0.

Original language	English
Pages (from-to)	247-251
Number of pages	5
Journal	Journal of Applied Biological Chemistry
Volume	58
Issue number	3
DOIs	https://doi.org/10.3839/jabc.2015.039
State	Published - 22 Sep 2015

Keywords

Chryseobacterium sp
Isolation
Keratin

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10.3839/jabc.2015.039

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title = "Isolation, identification, and characterization of a keratin-degrading bacterium chryseobacterium sp. P1-3",

abstract = "In this study, a keratin-degrading bacterium was isolated from soil contaminated with feather waste. The isolated strain was identified as Chryseobacterium sp. P1-3 on the basis of the 16S rRNA gene sequence alignment. Chryseobacterium sp. P1-3 is currently used in various biotechnological applications (e.g., in the hydrolysis of poultry feathers). It hydrolyzed the feather meal within 2 days and possesses a high level of keratinase activity (98 U/mL). The keratinase, partially purified from this strain, prefers casein as a substrate and shows optimal activity at a temperature of 30oC and at a pH of 8.0.",

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T1 - Isolation, identification, and characterization of a keratin-degrading bacterium chryseobacterium sp. P1-3

AU - Hong, Sung Jun

AU - Park, Gun Seok

AU - Jung, Byung Kwon

AU - Khan, Abdur Rahim

AU - Park, Yeong Jun

AU - Lee, Chang Hyun

AU - Shin, Jae Ho

PY - 2015/9/22

Y1 - 2015/9/22

N2 - In this study, a keratin-degrading bacterium was isolated from soil contaminated with feather waste. The isolated strain was identified as Chryseobacterium sp. P1-3 on the basis of the 16S rRNA gene sequence alignment. Chryseobacterium sp. P1-3 is currently used in various biotechnological applications (e.g., in the hydrolysis of poultry feathers). It hydrolyzed the feather meal within 2 days and possesses a high level of keratinase activity (98 U/mL). The keratinase, partially purified from this strain, prefers casein as a substrate and shows optimal activity at a temperature of 30oC and at a pH of 8.0.

AB - In this study, a keratin-degrading bacterium was isolated from soil contaminated with feather waste. The isolated strain was identified as Chryseobacterium sp. P1-3 on the basis of the 16S rRNA gene sequence alignment. Chryseobacterium sp. P1-3 is currently used in various biotechnological applications (e.g., in the hydrolysis of poultry feathers). It hydrolyzed the feather meal within 2 days and possesses a high level of keratinase activity (98 U/mL). The keratinase, partially purified from this strain, prefers casein as a substrate and shows optimal activity at a temperature of 30oC and at a pH of 8.0.

KW - Chryseobacterium sp

KW - Isolation

KW - Keratin

UR - https://www.scopus.com/pages/publications/84941949252

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DO - 10.3839/jabc.2015.039

M3 - Article

AN - SCOPUS:84941949252

SN - 1976-0442

VL - 58

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EP - 251

JO - Journal of Applied Biological Chemistry

JF - Journal of Applied Biological Chemistry

IS - 3

ER -

Isolation, identification, and characterization of a keratin-degrading bacterium chryseobacterium sp. P1-3

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Keywords

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