Isolation of melanin biosynthesis inhibitory compounds from the Phellodendri Cortex

Jong Gu Lee; Ji Young Choi; Joon Seok Oh; Hee Wook Jung; Eun Hyang Choi; Hee Sang Lee; Jeong Ah Kim; Tae Soo Chang; Jong Keun Son; Seung Ho Lee

Isolation of melanin biosynthesis inhibitory compounds from the Phellodendri Cortex

Jong Gu Lee, Ji Young Choi, Joon Seok Oh, Hee Wook Jung, Eun Hyang Choi, Hee Sang Lee, Jeong Ah Kim, Tae Soo Chang, Jong Keun Son, Seung Ho Lee

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

By screening inhibitory activities on the melanin polymer biosynthesis in B-16 mouse melanoma cell lines, MeOH extract of Phellodendri Cortex was found to have inhibitory effect on melanin polymer biosynthesis. Twelve compounds were isolated from the MeOH extract of P. Cortex. They were identified as obacunone (1), limonin (2), β-sitosterol (3), bis(2-methylheptyl) phthalate (4), cycloeucalenol (5), berberine (6), palmatine (7), jatrorrhizine (8), syringin (9), umbelliferone (10), rutaecarpine (11) and scopoletin (12) by comparison of their physical and spectral data with those of authentic samples. Among the isolated compounds, berberine (6) and palmatine (7) showed potent inhibitory effect on the melanin polymer biosynthesis in cultured B-16 mouse melanoma cell lines, with Inhibition rate of 96% and 90%, respectively. As a positive control, arbutin exhibited an inhibition rate of 56%.

Original language	English
Pages (from-to)	387-393
Number of pages	7
Journal	Korean Journal of Pharmacognosy
Volume	38
Issue number	4
State	Published - Dec 2007

Keywords

Berberine
Limonin
Melanin
Palmatine
Phellodendri cortex

Cite this

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title = "Isolation of melanin biosynthesis inhibitory compounds from the Phellodendri Cortex",

abstract = "By screening inhibitory activities on the melanin polymer biosynthesis in B-16 mouse melanoma cell lines, MeOH extract of Phellodendri Cortex was found to have inhibitory effect on melanin polymer biosynthesis. Twelve compounds were isolated from the MeOH extract of P. Cortex. They were identified as obacunone (1), limonin (2), β-sitosterol (3), bis(2-methylheptyl) phthalate (4), cycloeucalenol (5), berberine (6), palmatine (7), jatrorrhizine (8), syringin (9), umbelliferone (10), rutaecarpine (11) and scopoletin (12) by comparison of their physical and spectral data with those of authentic samples. Among the isolated compounds, berberine (6) and palmatine (7) showed potent inhibitory effect on the melanin polymer biosynthesis in cultured B-16 mouse melanoma cell lines, with Inhibition rate of 96% and 90%, respectively. As a positive control, arbutin exhibited an inhibition rate of 56%.",

keywords = "Berberine, Limonin, Melanin, Palmatine, Phellodendri cortex",

author = "Lee, {Jong Gu} and Choi, {Ji Young} and Oh, {Joon Seok} and Jung, {Hee Wook} and Choi, {Eun Hyang} and Lee, {Hee Sang} and Kim, {Jeong Ah} and Chang, {Tae Soo} and Son, {Jong Keun} and Lee, {Seung Ho}",

year = "2007",

month = dec,

language = "English",

volume = "38",

pages = "387--393",

journal = "Korean Journal of Pharmacognosy",

issn = "0253-3073",

publisher = "Korean Society of Pharmacognosy",

number = "4",

}

TY - JOUR

T1 - Isolation of melanin biosynthesis inhibitory compounds from the Phellodendri Cortex

AU - Lee, Jong Gu

AU - Choi, Ji Young

AU - Oh, Joon Seok

AU - Jung, Hee Wook

AU - Choi, Eun Hyang

AU - Lee, Hee Sang

AU - Kim, Jeong Ah

AU - Chang, Tae Soo

AU - Son, Jong Keun

AU - Lee, Seung Ho

PY - 2007/12

Y1 - 2007/12

N2 - By screening inhibitory activities on the melanin polymer biosynthesis in B-16 mouse melanoma cell lines, MeOH extract of Phellodendri Cortex was found to have inhibitory effect on melanin polymer biosynthesis. Twelve compounds were isolated from the MeOH extract of P. Cortex. They were identified as obacunone (1), limonin (2), β-sitosterol (3), bis(2-methylheptyl) phthalate (4), cycloeucalenol (5), berberine (6), palmatine (7), jatrorrhizine (8), syringin (9), umbelliferone (10), rutaecarpine (11) and scopoletin (12) by comparison of their physical and spectral data with those of authentic samples. Among the isolated compounds, berberine (6) and palmatine (7) showed potent inhibitory effect on the melanin polymer biosynthesis in cultured B-16 mouse melanoma cell lines, with Inhibition rate of 96% and 90%, respectively. As a positive control, arbutin exhibited an inhibition rate of 56%.

AB - By screening inhibitory activities on the melanin polymer biosynthesis in B-16 mouse melanoma cell lines, MeOH extract of Phellodendri Cortex was found to have inhibitory effect on melanin polymer biosynthesis. Twelve compounds were isolated from the MeOH extract of P. Cortex. They were identified as obacunone (1), limonin (2), β-sitosterol (3), bis(2-methylheptyl) phthalate (4), cycloeucalenol (5), berberine (6), palmatine (7), jatrorrhizine (8), syringin (9), umbelliferone (10), rutaecarpine (11) and scopoletin (12) by comparison of their physical and spectral data with those of authentic samples. Among the isolated compounds, berberine (6) and palmatine (7) showed potent inhibitory effect on the melanin polymer biosynthesis in cultured B-16 mouse melanoma cell lines, with Inhibition rate of 96% and 90%, respectively. As a positive control, arbutin exhibited an inhibition rate of 56%.

KW - Berberine

KW - Limonin

KW - Melanin

KW - Palmatine

KW - Phellodendri cortex

UR - http://www.scopus.com/inward/record.url?scp=76649125909&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:76649125909

SN - 0253-3073

VL - 38

SP - 387

EP - 393

JO - Korean Journal of Pharmacognosy

JF - Korean Journal of Pharmacognosy

IS - 4

ER -

Isolation of melanin biosynthesis inhibitory compounds from the Phellodendri Cortex

Abstract

Keywords

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