Isolation, sequencing, and characterization of the cytochrome bo operon from Vitreoscilla

The entire operon encoding the sodium pumping cytochrome bo from the bacterium Vitreoscilla was isolated and sequenced, and this sequence was analyzed by blast and hydropathy plots. There are fairly similar phylogenetic relationships which apply to all five proteins, but overall greater similarity to members of the gamma subdivision than the beta subdivision of the Proteobacteria. Hydropathy plots of all five Cyo proteins show near identity with those of the corresponding E. coli subunits, indicating that the similarity extends from sequence to structure. The operon appears to have a typical Shine-Dalgarno sequence, an E. coli-like promoter, and several possible binding sites for regulatory proteins. The Vitreoscilla Cyo B subunit (the probable Na⁺ pump) is almost identical to E. coli Cyo B at 18 key amino acids; thus, there are no obvious changes in Vitreoscilla Cyo B that hint at the details of its Na⁺ pumping ability.