TY - JOUR
T1 - Karyotype analysis using FISH (fluorescence in situ hybridization) in Fragaria
AU - Rae Rho, Il
AU - Hwang, Yoon Jung
AU - Lee, Hyung Il
AU - Lee, Choon Hwan
AU - Lim, Ki Byung
PY - 2012/3/1
Y1 - 2012/3/1
N2 - The karyotypes of Fragaria species, diploid (Fragaria vesca 'UC-01', 'Hawaii-30', Fragaria nilgerrensis 'Whiteberry') and octoploid (Fragaria×. ananassa 'Akihime'), were analyzed using a method based on fluorescence in situ hybridization (FISH), using ribosomal DNA probes (5S rDNA and 45S rDNA). All diploid species of F. vesca and F. nilgerrensis had 2. n=. 14 chromosomes, while F.×. ananassa, an octoploid species, were 2. n=. 56. The total length of somatic metaphase chromosome was measured as 12.22, 13.61, and 11.19μm for 'UC-01', 'Hawaii-30', and 'Whiteberry', respectively, and 'Akihime' measured 94.23μm. The karyotypes were composed of 6 pairs of metacentric and 1 pair of submetacentric in 'UC-01' and 'Whiteberry', and 5 pairs of metacentric and 2 pairs of submetacentric in 'Hawaii-30'. 5S rDNA loci were detected in 1 pair from 'UC-01', 'Hawaii-30' and 'Whiteberry', and 45S rDNA loci were detected in 3 pairs from 'UC-01' and 'Hawaii-30', whereas 45S rDNA loci were detected in 2 pairs from 'Whiteberry'. In octoploid strawberry 'Akihime', 45S rDNA signals were observed at 12 chromosomes, and 5S rDNA signals were observed at 2 chromosomes.
AB - The karyotypes of Fragaria species, diploid (Fragaria vesca 'UC-01', 'Hawaii-30', Fragaria nilgerrensis 'Whiteberry') and octoploid (Fragaria×. ananassa 'Akihime'), were analyzed using a method based on fluorescence in situ hybridization (FISH), using ribosomal DNA probes (5S rDNA and 45S rDNA). All diploid species of F. vesca and F. nilgerrensis had 2. n=. 14 chromosomes, while F.×. ananassa, an octoploid species, were 2. n=. 56. The total length of somatic metaphase chromosome was measured as 12.22, 13.61, and 11.19μm for 'UC-01', 'Hawaii-30', and 'Whiteberry', respectively, and 'Akihime' measured 94.23μm. The karyotypes were composed of 6 pairs of metacentric and 1 pair of submetacentric in 'UC-01' and 'Whiteberry', and 5 pairs of metacentric and 2 pairs of submetacentric in 'Hawaii-30'. 5S rDNA loci were detected in 1 pair from 'UC-01', 'Hawaii-30' and 'Whiteberry', and 45S rDNA loci were detected in 3 pairs from 'UC-01' and 'Hawaii-30', whereas 45S rDNA loci were detected in 2 pairs from 'Whiteberry'. In octoploid strawberry 'Akihime', 45S rDNA signals were observed at 12 chromosomes, and 5S rDNA signals were observed at 2 chromosomes.
KW - 45S rDNA
KW - 5S rDNA
KW - Chromosome
KW - FISH
KW - Strawberry
UR - http://www.scopus.com/inward/record.url?scp=84862793007&partnerID=8YFLogxK
U2 - 10.1016/j.scienta.2011.12.025
DO - 10.1016/j.scienta.2011.12.025
M3 - Article
AN - SCOPUS:84862793007
SN - 0304-4238
VL - 136
SP - 95
EP - 100
JO - Scientia Horticulturae
JF - Scientia Horticulturae
ER -