Korean paddy soil microbial community analysis method using denaturing gradient gel electrophoresis

Myeongeun Choe, Sung Jun Hong, Jong Hui Lim, Yunyoung Kwak, Chang Gi Back, Hee Young Jung, In Jung Lee, Jae Ho Shin

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Soil microbes are important integral components of soil ecosystem which have significant and diverse role in organic matter decomposition, nitrogen cycling, and nitrogen fixation. In this study an effective denaturing gradient gel electrophoresis (DGGE) method was employed for paddy soil microbial diversity survey. For optimum paddy soil microbial DNA extraction, different methods such as Lysis buffer, skim milk bead, sodium phosphate buffer, Epicentre Soil Master DNA extraction kit (Epicentre, USA) and Mo Bio Power Soil DNA kit (MO BIO, USA) methods were utilized. Among all the method, using Mo Bio Power Soil kit was most effective. DGGE analysis of Bacteria was carried out at 6% polyacylamide gel and 45-60% denaturing gradient in the optimal conditions. Whereas DGGE analysis of fungi was done at 6% polyacrylamide gel and 45-80% denaturing gradient in the optimal conditions. By applying the above assay, it was found that variation within the microbial community of paddy soil occurs by a factor of time. DGGE assay used in this study through for a variety of soil microbial analysis suggests the potential use of this method.

Original languageEnglish
Pages (from-to)95-100
Number of pages6
JournalJournal of Applied Biological Chemistry
Volume56
Issue number2
DOIs
StatePublished - 2013

Keywords

  • Denaturing gradient gel electrophoresis (DGGE)
  • Paddy soil microbial community

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