L arkinella humicola sp. nov., a gamma radiation-resistant bacterium isolated from soil

A Gram-negative, short rod-shaped, and pink-pigmented bacterial strain, designated MA1^T, was isolated from a soil sample from Gijang-gun, Busan in Republic of Korea. The 16S rRNA gene sequence analysis showed that strain MA1^T belonged to the genus Larkinella and was closely related to “Larkinella punicea” (97.5% similarity), Larkinella rosea 15J16-1T3A^T (96.5%), and Larkinella knui 15J6-3T6^T (96.2%). Polar lipid profile of strain MA1^T contained phosphatidylethanolamine, two unidentified aminolipids, and three unidentified lipids. Menaquinone-7 was the only quinone and the main fatty acids were C_16:1ω5c (36.7%), iso-C_15:0 (30.0%), iso-C_17:0 3-OH (7.7%), and summed feature 3 (C_16:1ω6c and/or C_16:1ω7c and/or iso-C_15:0 2-OH) (7.3%). The genomic DNA G + C content was 52.3 mol% based on the whole-genome analysis. Strain MA1^T exhibited a relatively low level of ANI and in silico DDH values with “Larkinella punicea” (91.9 and 47.1%, respectively), Larkinella rosea (79.7 and 23.3%), and Larkinella knui(81.9 and 25.7%). Based on its phenotypic properties and phylogenetic distinctiveness, strain MA1^T should be classified in the genus Larkinella as a representative of a novel species, for which the name Larkinella humicola sp. nov. is proposed. The type strain is MA1^T (= KCTC 72629^T = NBRC 114191^T).