TY - JOUR
T1 - Label- and enzyme-free detection of glucose by boronic acid-coupled poly(styrene-b-acrylic acid) at liquid crystal/aqueous interfaces
AU - Munir, Sundas
AU - Park, Soo Young
N1 - Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2018/11/22
Y1 - 2018/11/22
N2 - A glucose biosensor was developed based on a 4-cyano-4′-pentylbiphenyl (5CB)-filled transmission electron microscopy (TEM) grid cell by coating poly (styrene-b-acrylic acid) (PS-b-PAA) at the 5CB/aqueous interface and immobilising 3-aminophenyl boronic acid (APBA) on the PAA block chains by N-(3-dimethylaminopropyl)-N-ethylcarbodiimide coupling. Binding events between APBA and glucose were translated into homeotropic-to-planar configurational changes of 5CB, which were observed by polarised optical microscopy (POM) under crossed polarisers. This liquid-crystal-based glucose biosensor exhibited high sensitivity (limit of detection: 0.1 mM), even in complex serum and urine samples, high selectivity (against cholesterol, haemoglobin, and urea), and good stability for 40 d. This new and sensitive glucose biosensor has the advantages of low production cost, a simple immobilisation technique, and easy detection with POM, and may be useful for pre-screening glucose levels in human samples (serum and urine).
AB - A glucose biosensor was developed based on a 4-cyano-4′-pentylbiphenyl (5CB)-filled transmission electron microscopy (TEM) grid cell by coating poly (styrene-b-acrylic acid) (PS-b-PAA) at the 5CB/aqueous interface and immobilising 3-aminophenyl boronic acid (APBA) on the PAA block chains by N-(3-dimethylaminopropyl)-N-ethylcarbodiimide coupling. Binding events between APBA and glucose were translated into homeotropic-to-planar configurational changes of 5CB, which were observed by polarised optical microscopy (POM) under crossed polarisers. This liquid-crystal-based glucose biosensor exhibited high sensitivity (limit of detection: 0.1 mM), even in complex serum and urine samples, high selectivity (against cholesterol, haemoglobin, and urea), and good stability for 40 d. This new and sensitive glucose biosensor has the advantages of low production cost, a simple immobilisation technique, and easy detection with POM, and may be useful for pre-screening glucose levels in human samples (serum and urine).
KW - 3-Aminophenyl boronic acid
KW - Glucose
KW - Liquid crystal
KW - Non-enzymatic biosensor
KW - Polyelectrolyte brush
UR - http://www.scopus.com/inward/record.url?scp=85047353108&partnerID=8YFLogxK
U2 - 10.1016/j.aca.2018.05.039
DO - 10.1016/j.aca.2018.05.039
M3 - Article
C2 - 30143209
AN - SCOPUS:85047353108
SN - 0003-2670
VL - 1032
SP - 122
EP - 129
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
ER -