Larkinella harenae sp. nov., Isolated from Korean Beach Soil

A Gram-negative, short rod-shaped, gliding motile, and pale pink-pigmented bacterial strain, designated 15J9-9^T, was isolated from the soil of Iho Tewoo Beach, Jeju Island, Korea, and characterized taxonomically using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain 15J9-9^T belonged to the family Cytophagaceae and was related to Larkinella bovis M2T2B15^T (96.5% similarity), Larkinella arboricola Z0532^T (95.3% similarity), and Larkinella insperata LMG 22510^T (95.2% similarity). The DNA G+C content of strain 15J9-9^T was 48 mol%. The detection of phosphatidylethanolamine, phosphatidylserine, two unknown aminophospholipids (APL₁ and APL₂), and two unknown polar lipids (L₁ and L₂) in polar lipid profile, menaquinone-7 as the predominant quinone, and a fatty acid profile of C_16:1ω5c, iso-C_15:0, and iso-C_17:0 3-OH as the major fatty acids supported the affiliation of strain 15J9-9^T to the genus Larkinella. Based on its phenotypic properties and phylogenetic distinctiveness, strain 15J9-9^T should be classified in the genus Larkinella as a representative of a novel species, for which the name Larkinella harenae sp. nov. is proposed. The type strain is 15J9-9^T (=KCTC 42999^T = JCM 31656^T).