Abstract
A 4-cyano-4′-pentylbiphenyl (5CB)-filled transmission electron microscope (TEM) grid cell, which was functionalized by coating with a cationic surfactant dodecyltrimethylammonium bromide (DTAB) and the subsequent spontaneous adsorption of anionic deoxyribonucleic acid (DNA) at the liquid-crystal (LC)/aqueous interface was used for the detection of myricetin (MY) using a polarized optical microscope under crossed polarizers. The 5CB in contact with DTAB exhibited a homeotropic orientation, which subsequently changed to a planar one via electrostatic interactions between DNA and DTAB. When MY was injected into the TEM grid cell, the planar orientation changed to a homeotropic one due to the degradation of DNA by MY, which was confirmed by an electrophoresis method. The maximum adsorption of DNA (500 μg/mL) enabled the detection of 0.03 mM MY. The sensitivity of MY detection was enhanced in the presence of metallic salts of Fe and Cu. High stability, sensitivity, and ease of preparation of the TEM grid sensor make this new LC sensor suitable for the screening of trace amounts of MY.
| Original language | English |
|---|---|
| Pages (from-to) | 559-565 |
| Number of pages | 7 |
| Journal | Sensors and Actuators, B: Chemical |
| Volume | 233 |
| DOIs | |
| State | Published - 5 Oct 2016 |
Keywords
- Biosensor
- DNA
- DTAB
- Liquid crystal
- Myricetin