Loop-mediated isothermal amplification assay for the rapid and visual detection of novel porcine circovirus 3

Yu Ri Park; Hye Ryung Kim; Seong Hee Kim; Kyoung Ki Lee; Young S. Lyoo; Sang Geon Yeo; Choi Kyu Park

doi:10.1016/j.jviromet.2017.12.006

Loop-mediated isothermal amplification assay for the rapid and visual detection of novel porcine circovirus 3

Yu Ri Park, Hye Ryung Kim, Seong Hee Kim, Kyoung Ki Lee, Young S. Lyoo, Sang Geon Yeo, Choi Kyu Park

Department of Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

A loop-mediated isothermal amplification (LAMP) assay using hydroxynaphthol blue was developed for the rapid and visual detection of the capsid gene of porcine circovirus 3 (PCV3). The amplification could be completed in 40 min at 62°C, and the results could be visually detected by the naked eye. The assay specifically amplified PCV3 DNA and not other porcine viral nucleic acids. The limit of detection of the assay was 50 PCV3 DNA copies, which was comparable to that of the real-time polymerase chain reaction (qPCR) and lower than that of conventional PCR. In the clinical evaluation, the PCV3 detection rate of the LAMP assay was higher than that of PCR and agreed 100% with that of qPCR. These results indicate that the LAMP assay will be a valuable tool for the rapid, sensitive, and specific detection of PCV3 in clinical samples.

Original language	English
Pages (from-to)	26-30
Number of pages	5
Journal	Journal of Virological Methods
Volume	253
DOIs	https://doi.org/10.1016/j.jviromet.2017.12.006
State	Published - Mar 2018

Keywords

Capsid gene
Hydroxynaphthol blue
Loop-mediated isothermal amplification
Porcine circovirus 3

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10.1016/j.jviromet.2017.12.006

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title = "Loop-mediated isothermal amplification assay for the rapid and visual detection of novel porcine circovirus 3",

abstract = "A loop-mediated isothermal amplification (LAMP) assay using hydroxynaphthol blue was developed for the rapid and visual detection of the capsid gene of porcine circovirus 3 (PCV3). The amplification could be completed in 40 min at 62°C, and the results could be visually detected by the naked eye. The assay specifically amplified PCV3 DNA and not other porcine viral nucleic acids. The limit of detection of the assay was 50 PCV3 DNA copies, which was comparable to that of the real-time polymerase chain reaction (qPCR) and lower than that of conventional PCR. In the clinical evaluation, the PCV3 detection rate of the LAMP assay was higher than that of PCR and agreed 100% with that of qPCR. These results indicate that the LAMP assay will be a valuable tool for the rapid, sensitive, and specific detection of PCV3 in clinical samples.",

keywords = "Capsid gene, Hydroxynaphthol blue, Loop-mediated isothermal amplification, Porcine circovirus 3",

author = "Park, {Yu Ri} and Kim, {Hye Ryung} and Kim, {Seong Hee} and Lee, {Kyoung Ki} and Lyoo, {Young S.} and Yeo, {Sang Geon} and Park, {Choi Kyu}",

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year = "2018",

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doi = "10.1016/j.jviromet.2017.12.006",

language = "English",

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T1 - Loop-mediated isothermal amplification assay for the rapid and visual detection of novel porcine circovirus 3

AU - Park, Yu Ri

AU - Kim, Hye Ryung

AU - Kim, Seong Hee

AU - Lee, Kyoung Ki

AU - Lyoo, Young S.

AU - Yeo, Sang Geon

AU - Park, Choi Kyu

PY - 2018/3

Y1 - 2018/3

N2 - A loop-mediated isothermal amplification (LAMP) assay using hydroxynaphthol blue was developed for the rapid and visual detection of the capsid gene of porcine circovirus 3 (PCV3). The amplification could be completed in 40 min at 62°C, and the results could be visually detected by the naked eye. The assay specifically amplified PCV3 DNA and not other porcine viral nucleic acids. The limit of detection of the assay was 50 PCV3 DNA copies, which was comparable to that of the real-time polymerase chain reaction (qPCR) and lower than that of conventional PCR. In the clinical evaluation, the PCV3 detection rate of the LAMP assay was higher than that of PCR and agreed 100% with that of qPCR. These results indicate that the LAMP assay will be a valuable tool for the rapid, sensitive, and specific detection of PCV3 in clinical samples.

AB - A loop-mediated isothermal amplification (LAMP) assay using hydroxynaphthol blue was developed for the rapid and visual detection of the capsid gene of porcine circovirus 3 (PCV3). The amplification could be completed in 40 min at 62°C, and the results could be visually detected by the naked eye. The assay specifically amplified PCV3 DNA and not other porcine viral nucleic acids. The limit of detection of the assay was 50 PCV3 DNA copies, which was comparable to that of the real-time polymerase chain reaction (qPCR) and lower than that of conventional PCR. In the clinical evaluation, the PCV3 detection rate of the LAMP assay was higher than that of PCR and agreed 100% with that of qPCR. These results indicate that the LAMP assay will be a valuable tool for the rapid, sensitive, and specific detection of PCV3 in clinical samples.

KW - Capsid gene

KW - Hydroxynaphthol blue

KW - Loop-mediated isothermal amplification

KW - Porcine circovirus 3

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SP - 26

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JO - Journal of Virological Methods

JF - Journal of Virological Methods

ER -

Loop-mediated isothermal amplification assay for the rapid and visual detection of novel porcine circovirus 3

Abstract

Keywords

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