Loop-mediated isothermal amplification targeting actin DNA of Trichomonas vaginalis

Youn Kyoung Goo, Won Sik Shin, Hye Won Yang, So Young Joo, Su Min Song, Jae Sook Ryu, Hyun Hee Kong, Won Ki Lee, Dong Il Chung, Yeonchul Hong

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Trichomoniasis caused by Trichomonas vaginalis is a common sexually transmitted disease. Its association with several health problems, including preterm birth, pelvic inflammatory disease, cervical cancer, and transmission of human immunodeficiency virus, emphasizes the importance of improved access to early and accurate detection of T. vaginalis. In this study, a rapid and efficient loop-mediated isothermal amplification-based method for the detection of T. vaginalis was developed and validated, using vaginal swab specimens from subjects suspected to have trichomoniasis. The LAMP assay targeting the actin gene was highly sensitive with detection limits of 1 trichomonad and 1 pg of T. vaginalis DNA per reaction, and specifically amplified the target gene only from T. vaginalis. Validation of this assay showed that it had the highest sensitivity and better agreement with PCR (used as the gold standard) compared to microscopy and multiplex PCR. This study showed that the LAMP assay, targeting the actin gene, could be used to diagnose early infections of T. vaginalis. Thus, we have provided an alternative molecular diagnostic tool and a point-of-care test that may help to prevent trichomoniasis transmission and associated complications.

Original languageEnglish
Pages (from-to)329-334
Number of pages6
JournalKorean Journal of Parasitology
Volume54
Issue number3
DOIs
StatePublished - Jun 2016

Keywords

  • Loop-mediated isothermal amplification
  • Multiplex PCR
  • PCR
  • Trichomonas vaginalis
  • Trichomoniasis

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