Loss of Arabidopsis PATATIN-RELATED PHOSPHOLIPASE 2A Affects the Expression of Genes Involved in Fertilization

Jun Soo Kwak; Jong Tae Song; Hak Soo Seo

doi:10.9787/PBB.2022.10.1.49

Loss of Arabidopsis PATATIN-RELATED PHOSPHOLIPASE 2A Affects the Expression of Genes Involved in Fertilization

Jun Soo Kwak, Jong Tae Song, Hak Soo Seo

Seoul National University

Research output: Contribution to journal › Article › peer-review

Abstract

Doubled haploid (DH) technology enables the generation and evaluation of new plant genotypes in ≤ 2 years, which is in stark contrast to the 6-7 years required for conventional breeding. Recently, a few proteins including MATRILINEAL (MTL) were found to trigger haploid induction in monocot plants. However, MTL function in dicot plants remains unclear. Here, we investigate the effects of Arabidopsis PATATIN-RELATED PHOSPHOLIPASE 2A (pPLA-IIα), a maize MTL homolog, on the expression of genes that modulate pollen development and fertilization in Arabidopsis. Quantitative real-time PCR analysis showed that transcript levels of four pollen tube growth-related genes and six pollen guidance or reception-related genes were increased in the ppla-iiα T-DNA insertion mutant compared with the wild type. In addition, transcript levels of four mitosis and meiosis-related genes, two hormone-related genes, and a calcium pump-encoding gene were also upregulated in the ppla-iiα mutant, whereas expression levels of genes encoding a synergid-secreted peptide (AtLURE1.1) and calmodulin-activated Ca ^2＋ -ATPase ion pump 9 (ACA9) protein were downregulated in the ppla-iiα mutant compared with the wild type. Taken together, these results suggest that AtLURE1.1 and ACA9 play positive roles in pPLA-IIα-mediated fertilization. Thus, pPLA-IIα controls fertilization through the modulation of pollen germination and pollen tube growth and guidance in Arabidopsis.

Original language	English
Pages (from-to)	49-61
Number of pages	13
Journal	Plant Breeding and Biotechnology
Volume	10
Issue number	1
DOIs	https://doi.org/10.9787/PBB.2022.10.1.49
State	Published - Mar 2022

Keywords

Fertilization
Haploid
Patatin-like phophpolipase 2a
Pollen tube
Ppla-iiα
Synergid

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10.9787/PBB.2022.10.1.49

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title = "Loss of Arabidopsis PATATIN-RELATED PHOSPHOLIPASE 2A Affects the Expression of Genes Involved in Fertilization",

abstract = "Doubled haploid (DH) technology enables the generation and evaluation of new plant genotypes in ≤ 2 years, which is in stark contrast to the 6-7 years required for conventional breeding. Recently, a few proteins including MATRILINEAL (MTL) were found to trigger haploid induction in monocot plants. However, MTL function in dicot plants remains unclear. Here, we investigate the effects of Arabidopsis PATATIN-RELATED PHOSPHOLIPASE 2A (pPLA-IIα), a maize MTL homolog, on the expression of genes that modulate pollen development and fertilization in Arabidopsis. Quantitative real-time PCR analysis showed that transcript levels of four pollen tube growth-related genes and six pollen guidance or reception-related genes were increased in the ppla-iiα T-DNA insertion mutant compared with the wild type. In addition, transcript levels of four mitosis and meiosis-related genes, two hormone-related genes, and a calcium pump-encoding gene were also upregulated in the ppla-iiα mutant, whereas expression levels of genes encoding a synergid-secreted peptide (AtLURE1.1) and calmodulin-activated Ca 2＋ -ATPase ion pump 9 (ACA9) protein were downregulated in the ppla-iiα mutant compared with the wild type. Taken together, these results suggest that AtLURE1.1 and ACA9 play positive roles in pPLA-IIα-mediated fertilization. Thus, pPLA-IIα controls fertilization through the modulation of pollen germination and pollen tube growth and guidance in Arabidopsis.",

keywords = "Fertilization, Haploid, Patatin-like phophpolipase 2a, Pollen tube, Ppla-iiα, Synergid",

author = "Kwak, {Jun Soo} and Song, {Jong Tae} and Seo, {Hak Soo}",

note = "Publisher Copyright: {\textcopyright} 2022 by the Korean Society of Breeding Science This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.",

year = "2022",

month = mar,

doi = "10.9787/PBB.2022.10.1.49",

language = "English",

volume = "10",

pages = "49--61",

journal = "Plant Breeding and Biotechnology",

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AU - Kwak, Jun Soo

AU - Song, Jong Tae

AU - Seo, Hak Soo

N1 - Publisher Copyright: © 2022 by the Korean Society of Breeding Science This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PY - 2022/3

Y1 - 2022/3

N2 - Doubled haploid (DH) technology enables the generation and evaluation of new plant genotypes in ≤ 2 years, which is in stark contrast to the 6-7 years required for conventional breeding. Recently, a few proteins including MATRILINEAL (MTL) were found to trigger haploid induction in monocot plants. However, MTL function in dicot plants remains unclear. Here, we investigate the effects of Arabidopsis PATATIN-RELATED PHOSPHOLIPASE 2A (pPLA-IIα), a maize MTL homolog, on the expression of genes that modulate pollen development and fertilization in Arabidopsis. Quantitative real-time PCR analysis showed that transcript levels of four pollen tube growth-related genes and six pollen guidance or reception-related genes were increased in the ppla-iiα T-DNA insertion mutant compared with the wild type. In addition, transcript levels of four mitosis and meiosis-related genes, two hormone-related genes, and a calcium pump-encoding gene were also upregulated in the ppla-iiα mutant, whereas expression levels of genes encoding a synergid-secreted peptide (AtLURE1.1) and calmodulin-activated Ca 2＋ -ATPase ion pump 9 (ACA9) protein were downregulated in the ppla-iiα mutant compared with the wild type. Taken together, these results suggest that AtLURE1.1 and ACA9 play positive roles in pPLA-IIα-mediated fertilization. Thus, pPLA-IIα controls fertilization through the modulation of pollen germination and pollen tube growth and guidance in Arabidopsis.

AB - Doubled haploid (DH) technology enables the generation and evaluation of new plant genotypes in ≤ 2 years, which is in stark contrast to the 6-7 years required for conventional breeding. Recently, a few proteins including MATRILINEAL (MTL) were found to trigger haploid induction in monocot plants. However, MTL function in dicot plants remains unclear. Here, we investigate the effects of Arabidopsis PATATIN-RELATED PHOSPHOLIPASE 2A (pPLA-IIα), a maize MTL homolog, on the expression of genes that modulate pollen development and fertilization in Arabidopsis. Quantitative real-time PCR analysis showed that transcript levels of four pollen tube growth-related genes and six pollen guidance or reception-related genes were increased in the ppla-iiα T-DNA insertion mutant compared with the wild type. In addition, transcript levels of four mitosis and meiosis-related genes, two hormone-related genes, and a calcium pump-encoding gene were also upregulated in the ppla-iiα mutant, whereas expression levels of genes encoding a synergid-secreted peptide (AtLURE1.1) and calmodulin-activated Ca 2＋ -ATPase ion pump 9 (ACA9) protein were downregulated in the ppla-iiα mutant compared with the wild type. Taken together, these results suggest that AtLURE1.1 and ACA9 play positive roles in pPLA-IIα-mediated fertilization. Thus, pPLA-IIα controls fertilization through the modulation of pollen germination and pollen tube growth and guidance in Arabidopsis.

KW - Fertilization

KW - Haploid

KW - Patatin-like phophpolipase 2a

KW - Pollen tube

KW - Ppla-iiα

KW - Synergid

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SN - 2287-9358

VL - 10

SP - 49

EP - 61

JO - Plant Breeding and Biotechnology

JF - Plant Breeding and Biotechnology

IS - 1

ER -

Loss of Arabidopsis PATATIN-RELATED PHOSPHOLIPASE 2A Affects the Expression of Genes Involved in Fertilization

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