Low-cost, high-efficiency double-sided neural probe

Neural probes are indispensable in neuroscience research, enabling precise neuronal spike signal recording and electrical stimulation. Advancements in brain-computer interface technologies and the growing need to study neurological disorders have driven the increasing demand for accurate neural signal recording. While dual-sided probes address this demand, their scalability is limited by complex fabrication processes and high production costs. This study introduces a flexible, dual-sided neural probe that combines stable performance, cost efficiency (∼$3.50 per unit) and rapid fabrication (∼7 days). Fabricated using commercially available flexible printed circuit board processes, the proposed probe simplifies packaging steps, making it well-suited for mass production. The probe, equipped with 16 dual-sided electrodes positioned on 4 shanks, successfully recorded neural signals across up to four brain regions, demonstrating high accuracy in both single- and multi-region recordings. The overall reduction in probe thickness was achieved by integrating photo solder resist (PSR) ink insulation, which helps minimize brain tissue damage. This cost-effective, scalable dual-sided probe holds significant potential for seamless integration with miniaturized wireless systems, and brain-computer interface (BCI) applications. Its enhanced accessibility and broader applicability in neuroscience research highlight its potential impact on the field.