Low density lipoprotein-oxidation inhibitory phytochemicals from the fruits of rhus parviflora

Fruits of Rhus parviflora were extracted with 80% aqueous methanol (MeOH), and the concentrated extract was partitioned using ethyl acetate (EtOAc), n-butanol (n-BuOH), and H₂O, successively. Purification of EtOAc fraction led to isolation of fifteen polyphenols of which structures were identified by spectroscopic methods including 2D-NMR. Most compounds apart from compound 10 inhibited low density lipoproteinoxidation within IC₅₀ value of 10 µM. Among compounds, taxifolin (2), quercetin 3-O-α-L-rhamnopyranoside (13), agathisflavone (5) sulfuretin (4), and aureusidin (3) showed IC₅₀ values 0.9, 0.8, 5.8, 2.9, and 2.4 µM which were of highly significant in comparison positive control butylated hydroxytoluene with IC₅₀ value of 2.1 µM. The results indicate fruits of R. parviflora as a source of antihypercholesterolemic compounds.