Low SP1 Expression Differentially Affects Intestinal-Type Compared with Diffuse-Type Gastric Adenocarcinoma

Hun Seok Lee; Cheol Keun Park; Ensel Oh; Özgür Cem Erkin; Hun Soon Jung; Mi Hyun Cho; Mi Jeong Kwon; Seoung Wan Chae; Seok Hyung Kim; Li Hui Wang; Min Jeong Park; Su Yeon Lee; Ho Bin Yang; Lina Jia; Yoon La Choi; Young Kee Shin

doi:10.1371/journal.pone.0055522

Low SP1 Expression Differentially Affects Intestinal-Type Compared with Diffuse-Type Gastric Adenocarcinoma

Hun Seok Lee, Cheol Keun Park, Ensel Oh, Özgür Cem Erkin, Hun Soon Jung, Mi Hyun Cho, Mi Jeong Kwon, Seoung Wan Chae, Seok Hyung Kim, Li Hui Wang, Min Jeong Park, Su Yeon Lee, Ho Bin Yang, Lina Jia, Yoon La Choi, Young Kee Shin

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Specificity protein 1 (SP1) is an essential transcription factor that regulates multiple cancer-related genes. Because aberrant expression of SP1 is related to cancer development and progression, we focused on SP1 expression in gastric carcinoma and its correlation with disease outcomes. Although patient survival decreased as SP1 expression increased (P<0.05) in diffuse-type gastric cancer, the lack of SP1 expression in intestinal-type gastric cancer was significantly correlated with poor survival (P<0.05). The knockdown of SP1 in a high SP1-expressing intestinal-type gastric cell line, MKN28, increased migration and invasion but decreased proliferation. Microarray data in SP1 siRNA-transfected MKN28 revealed that the genes inhibiting migration were downregulated, whereas the genes negatively facilitating proliferation were increased. However, both migration and invasion were decreased by forced SP1 expression in a low SP1-expressing intestinal-type gastric cell line, AGS. Unlike the intestinal-type, in a high SP1-expressing diffuse-type gastric cell line, SNU484, migration and invasion were decreased by SP1 siRNA. In contrast to previous studies that did not identify differences between the 2 histological types, our results reveal that low expression of SP1 is involved in cancer progression and metastasis and differentially affects intestinal-type compared with diffuse-type gastric adenocarcinoma.

Original language	English
Article number	e55522
Journal	PLoS ONE
Volume	8
Issue number	2
DOIs	https://doi.org/10.1371/journal.pone.0055522
State	Published - 20 Feb 2013

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Lee, H. S., Park, C. K., Oh, E., Erkin, Ö. C., Jung, H. S., Cho, M. H., Kwon, M. J., Chae, S. W., Kim, S. H., Wang, L. H., Park, M. J., Lee, S. Y., Yang, H. B., Jia, L., Choi, Y. L., & Shin, Y. K. (2013). Low SP1 Expression Differentially Affects Intestinal-Type Compared with Diffuse-Type Gastric Adenocarcinoma. PLoS ONE, 8(2), Article e55522. https://doi.org/10.1371/journal.pone.0055522

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abstract = "Specificity protein 1 (SP1) is an essential transcription factor that regulates multiple cancer-related genes. Because aberrant expression of SP1 is related to cancer development and progression, we focused on SP1 expression in gastric carcinoma and its correlation with disease outcomes. Although patient survival decreased as SP1 expression increased (P<0.05) in diffuse-type gastric cancer, the lack of SP1 expression in intestinal-type gastric cancer was significantly correlated with poor survival (P<0.05). The knockdown of SP1 in a high SP1-expressing intestinal-type gastric cell line, MKN28, increased migration and invasion but decreased proliferation. Microarray data in SP1 siRNA-transfected MKN28 revealed that the genes inhibiting migration were downregulated, whereas the genes negatively facilitating proliferation were increased. However, both migration and invasion were decreased by forced SP1 expression in a low SP1-expressing intestinal-type gastric cell line, AGS. Unlike the intestinal-type, in a high SP1-expressing diffuse-type gastric cell line, SNU484, migration and invasion were decreased by SP1 siRNA. In contrast to previous studies that did not identify differences between the 2 histological types, our results reveal that low expression of SP1 is involved in cancer progression and metastasis and differentially affects intestinal-type compared with diffuse-type gastric adenocarcinoma.",

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AU - Erkin, Özgür Cem

AU - Jung, Hun Soon

AU - Cho, Mi Hyun

AU - Kwon, Mi Jeong

AU - Chae, Seoung Wan

AU - Kim, Seok Hyung

AU - Wang, Li Hui

AU - Park, Min Jeong

AU - Lee, Su Yeon

AU - Yang, Ho Bin

AU - Jia, Lina

AU - Choi, Yoon La

AU - Shin, Young Kee

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AB - Specificity protein 1 (SP1) is an essential transcription factor that regulates multiple cancer-related genes. Because aberrant expression of SP1 is related to cancer development and progression, we focused on SP1 expression in gastric carcinoma and its correlation with disease outcomes. Although patient survival decreased as SP1 expression increased (P<0.05) in diffuse-type gastric cancer, the lack of SP1 expression in intestinal-type gastric cancer was significantly correlated with poor survival (P<0.05). The knockdown of SP1 in a high SP1-expressing intestinal-type gastric cell line, MKN28, increased migration and invasion but decreased proliferation. Microarray data in SP1 siRNA-transfected MKN28 revealed that the genes inhibiting migration were downregulated, whereas the genes negatively facilitating proliferation were increased. However, both migration and invasion were decreased by forced SP1 expression in a low SP1-expressing intestinal-type gastric cell line, AGS. Unlike the intestinal-type, in a high SP1-expressing diffuse-type gastric cell line, SNU484, migration and invasion were decreased by SP1 siRNA. In contrast to previous studies that did not identify differences between the 2 histological types, our results reveal that low expression of SP1 is involved in cancer progression and metastasis and differentially affects intestinal-type compared with diffuse-type gastric adenocarcinoma.

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Low SP1 Expression Differentially Affects Intestinal-Type Compared with Diffuse-Type Gastric Adenocarcinoma

Abstract

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