MAL and TMEM220 are novel DNA methylation markers in human gastric cancer

Boram Choi; Tae Su Han; Jimin Min; Keun Hur; Sun Min Lee; Hyuk Joon Lee; Young Joon Kim; Han Kwang Yang

doi:10.1080/1354750X.2016.1201542

MAL and TMEM220 are novel DNA methylation markers in human gastric cancer

Boram Choi, Tae Su Han, Jimin Min, Keun Hur, Sun Min Lee, Hyuk Joon Lee, Young Joon Kim, Han Kwang Yang

Department of Medicine

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Context: Gastric cancer (GC) is the fourth most common cause of cancer-related deaths worldwide. Objective: To determine the mRNA-expression of the MAL, TMEM220, MMP28, IL-19 and HOPX genes and analyse the methylation statuses of MAL and TMEM220. Materials and methods: Gene-expression levels were analysed in 10 GC cell lines and 30 matched pairs of GC and normal mucosa (NM) gastric tissue specimens in real-time reverse-transcriptase polymerase chain reactions. Gene methylation was evaluated by bisulphite sequencing. Detailed gene-methylation patterns were confirmed by pyrosequencing analysis. Results:MAL, TMEM220, MMP28 and IL-19 were significantly down-regulated in GC cell lines and GC tissues compared to NM tissues. MAL and TMEM220 were highly methylated in GC tissues, and methylation inversely correlated with expression. MAL and TMEM220 expression were restored by treatment with 5-aza-2′-deoxycytidine. MAL and TMEM220 were specifically methylated and were down-regulated in human GC. Discussion and conclusion: These loci may serve as novel methylation markers for patients with GC.

Original language	English
Pages (from-to)	35-44
Number of pages	10
Journal	Biomarkers
Volume	22
Issue number	1
DOIs	https://doi.org/10.1080/1354750X.2016.1201542
State	Published - 2 Jan 2017

Keywords

Biomarker
gastric cancer methylation
HOPX
IL-19
MMP28
T-cell differentiation protein

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1080/1354750X.2016.1201542

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title = "MAL and TMEM220 are novel DNA methylation markers in human gastric cancer",

abstract = "Context: Gastric cancer (GC) is the fourth most common cause of cancer-related deaths worldwide. Objective: To determine the mRNA-expression of the MAL, TMEM220, MMP28, IL-19 and HOPX genes and analyse the methylation statuses of MAL and TMEM220. Materials and methods: Gene-expression levels were analysed in 10 GC cell lines and 30 matched pairs of GC and normal mucosa (NM) gastric tissue specimens in real-time reverse-transcriptase polymerase chain reactions. Gene methylation was evaluated by bisulphite sequencing. Detailed gene-methylation patterns were confirmed by pyrosequencing analysis. Results:MAL, TMEM220, MMP28 and IL-19 were significantly down-regulated in GC cell lines and GC tissues compared to NM tissues. MAL and TMEM220 were highly methylated in GC tissues, and methylation inversely correlated with expression. MAL and TMEM220 expression were restored by treatment with 5-aza-2′-deoxycytidine. MAL and TMEM220 were specifically methylated and were down-regulated in human GC. Discussion and conclusion: These loci may serve as novel methylation markers for patients with GC.",

keywords = "Biomarker, gastric cancer methylation, HOPX, IL-19, MMP28, T-cell differentiation protein",

author = "Boram Choi and Han, {Tae Su} and Jimin Min and Keun Hur and Lee, {Sun Min} and Lee, {Hyuk Joon} and Kim, {Young Joon} and Yang, {Han Kwang}",

note = "Publisher Copyright: {\textcopyright} 2016 Informa UK Limited, trading as Taylor & Francis Group.",

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doi = "10.1080/1354750X.2016.1201542",

language = "English",

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T1 - MAL and TMEM220 are novel DNA methylation markers in human gastric cancer

AU - Choi, Boram

AU - Han, Tae Su

AU - Min, Jimin

AU - Hur, Keun

AU - Lee, Sun Min

AU - Lee, Hyuk Joon

AU - Kim, Young Joon

AU - Yang, Han Kwang

PY - 2017/1/2

Y1 - 2017/1/2

N2 - Context: Gastric cancer (GC) is the fourth most common cause of cancer-related deaths worldwide. Objective: To determine the mRNA-expression of the MAL, TMEM220, MMP28, IL-19 and HOPX genes and analyse the methylation statuses of MAL and TMEM220. Materials and methods: Gene-expression levels were analysed in 10 GC cell lines and 30 matched pairs of GC and normal mucosa (NM) gastric tissue specimens in real-time reverse-transcriptase polymerase chain reactions. Gene methylation was evaluated by bisulphite sequencing. Detailed gene-methylation patterns were confirmed by pyrosequencing analysis. Results:MAL, TMEM220, MMP28 and IL-19 were significantly down-regulated in GC cell lines and GC tissues compared to NM tissues. MAL and TMEM220 were highly methylated in GC tissues, and methylation inversely correlated with expression. MAL and TMEM220 expression were restored by treatment with 5-aza-2′-deoxycytidine. MAL and TMEM220 were specifically methylated and were down-regulated in human GC. Discussion and conclusion: These loci may serve as novel methylation markers for patients with GC.

AB - Context: Gastric cancer (GC) is the fourth most common cause of cancer-related deaths worldwide. Objective: To determine the mRNA-expression of the MAL, TMEM220, MMP28, IL-19 and HOPX genes and analyse the methylation statuses of MAL and TMEM220. Materials and methods: Gene-expression levels were analysed in 10 GC cell lines and 30 matched pairs of GC and normal mucosa (NM) gastric tissue specimens in real-time reverse-transcriptase polymerase chain reactions. Gene methylation was evaluated by bisulphite sequencing. Detailed gene-methylation patterns were confirmed by pyrosequencing analysis. Results:MAL, TMEM220, MMP28 and IL-19 were significantly down-regulated in GC cell lines and GC tissues compared to NM tissues. MAL and TMEM220 were highly methylated in GC tissues, and methylation inversely correlated with expression. MAL and TMEM220 expression were restored by treatment with 5-aza-2′-deoxycytidine. MAL and TMEM220 were specifically methylated and were down-regulated in human GC. Discussion and conclusion: These loci may serve as novel methylation markers for patients with GC.

KW - Biomarker

KW - gastric cancer methylation

KW - HOPX

KW - IL-19

KW - MMP28

KW - T-cell differentiation protein

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SN - 1354-750X

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MAL and TMEM220 are novel DNA methylation markers in human gastric cancer

Abstract

Keywords

UN SDGs

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