Measuring primate RNFL thickness with OCT

We report results of a study using optical coherence tomography (OCT) to create area maps of the retinal nerve fiber layer (RNFL) thickness. Low-resolution B scans (two-dimensional images) of the in vivo rhesus monkey retina have been recorded in the optic disk, peripapillary area, and macula. Three-dimensional (3-D) renderings of these areas have been created from OCT images and compared to corresponding data from histology. Results of our study indicate: 1) OCT may be utilized to measure the retinal nerve fiber layer thickness in vivo and 2) 3-D surface maps accurately depict the anatomy of the RNFL.