Melittin induces apoptotic features in Candida albicans

Cana Park; Dong Gun Lee

doi:10.1016/j.bbrc.2010.02.138

Melittin induces apoptotic features in Candida albicans

Cana Park, Dong Gun Lee

Kyungpook National University

Research output: Contribution to journal › Article › peer-review

97 Scopus citations

Abstract

Melittin is a well-known antimicrobial peptide with membrane-active mechanisms. In this study, it was found that Melittin exerted its antifungal effect via apoptosis. Candida albicans exposed to Melittin showed the increased reactive oxygen species (ROS) production, measured by DHR-123 staining. Fluorescence microscopy staining with FITC-annexin V, TUNEL and DAPI further confirmed diagnostic markers of yeast apoptosis including phosphatidylserine externalization, and DNA and nuclear fragmentation. The current study suggests that Melittin possesses an antifungal effect with another mechanism promoting apoptosis.

Original language	English
Pages (from-to)	170-172
Number of pages	3
Journal	Biochemical and Biophysical Research Communications
Volume	394
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2010.02.138
State	Published - 26 Mar 2010

Keywords

Antifungal effect
Antimicrobial peptide
Apoptosis
Melittin

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10.1016/j.bbrc.2010.02.138

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AU - Lee, Dong Gun

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AB - Melittin is a well-known antimicrobial peptide with membrane-active mechanisms. In this study, it was found that Melittin exerted its antifungal effect via apoptosis. Candida albicans exposed to Melittin showed the increased reactive oxygen species (ROS) production, measured by DHR-123 staining. Fluorescence microscopy staining with FITC-annexin V, TUNEL and DAPI further confirmed diagnostic markers of yeast apoptosis including phosphatidylserine externalization, and DNA and nuclear fragmentation. The current study suggests that Melittin possesses an antifungal effect with another mechanism promoting apoptosis.

KW - Antifungal effect

KW - Antimicrobial peptide

KW - Apoptosis

KW - Melittin

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Melittin induces apoptotic features in Candida albicans

Abstract

Keywords

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