Mesenchymal stem cells contribute to improvement of renal function in a canine kidney injury model

Seung Jun Lee; Min Ok Ryu; Min Soo Seo; Sang Bum Park; Jin Ok Ahn; Sei Myoung Han; Kyung Sun Kang; Dong Ha Bhang; Hwa Young Youn

doi:10.21873/invivo.11177

Mesenchymal stem cells contribute to improvement of renal function in a canine kidney injury model

Seung Jun Lee, Min Ok Ryu, Min Soo Seo, Sang Bum Park, Jin Ok Ahn, Sei Myoung Han, Kyung Sun Kang, Dong Ha Bhang, Hwa Young Youn

Department of Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Background/Aim: The kidney excretes waste materials and regulates important metabolic functions, and renal disorders constitute a significant medical problem and can result in fatalities. In the present study, mesenchymal stem cells derived from canine umbilical cord blood (cUCB-MSCs) were isolated and evaluated for their ability to improve renal function in a canine model of acute kidney injury (AKI). Materials and Methods: The canine AKI model was developed by i.v. injection of cisplatin and gentamycin into 14 male beagle dogs. cUCB-MSCs were administered into the renal corticomedullary junction following AKI induction. Survival time, clinical signs, blood analysis and histological parameters were analyzed. Results: The group treated with AKI plus cUCB-MSCs had decreased blood urea nitrogen and creatinine levels, and showed an extended lifespan and improved histological manifestations. MSCs were detected around the tubules of these kidneys at the histological level. Conclusion: Taken together, our findings suggest that cUCB-MSCs could be an alternative therapeutic agent for canine AKI.

Original language	English
Pages (from-to)	1115-1124
Number of pages	10
Journal	In Vivo
Volume	31
Issue number	6
DOIs	https://doi.org/10.21873/invivo.11177
State	Published - 1 Nov 2017

Keywords

Acute kidney injury
Canine umbilical cord blood-mesenchymal stem cells
Dog

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.21873/invivo.11177

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title = "Mesenchymal stem cells contribute to improvement of renal function in a canine kidney injury model",

abstract = "Background/Aim: The kidney excretes waste materials and regulates important metabolic functions, and renal disorders constitute a significant medical problem and can result in fatalities. In the present study, mesenchymal stem cells derived from canine umbilical cord blood (cUCB-MSCs) were isolated and evaluated for their ability to improve renal function in a canine model of acute kidney injury (AKI). Materials and Methods: The canine AKI model was developed by i.v. injection of cisplatin and gentamycin into 14 male beagle dogs. cUCB-MSCs were administered into the renal corticomedullary junction following AKI induction. Survival time, clinical signs, blood analysis and histological parameters were analyzed. Results: The group treated with AKI plus cUCB-MSCs had decreased blood urea nitrogen and creatinine levels, and showed an extended lifespan and improved histological manifestations. MSCs were detected around the tubules of these kidneys at the histological level. Conclusion: Taken together, our findings suggest that cUCB-MSCs could be an alternative therapeutic agent for canine AKI.",

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author = "Lee, {Seung Jun} and Ryu, {Min Ok} and Seo, {Min Soo} and Park, {Sang Bum} and Ahn, {Jin Ok} and Han, {Sei Myoung} and Kang, {Kyung Sun} and Bhang, {Dong Ha} and Youn, {Hwa Young}",

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doi = "10.21873/invivo.11177",

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AU - Ryu, Min Ok

AU - Seo, Min Soo

AU - Park, Sang Bum

AU - Ahn, Jin Ok

AU - Han, Sei Myoung

AU - Kang, Kyung Sun

AU - Bhang, Dong Ha

AU - Youn, Hwa Young

PY - 2017/11/1

Y1 - 2017/11/1

N2 - Background/Aim: The kidney excretes waste materials and regulates important metabolic functions, and renal disorders constitute a significant medical problem and can result in fatalities. In the present study, mesenchymal stem cells derived from canine umbilical cord blood (cUCB-MSCs) were isolated and evaluated for their ability to improve renal function in a canine model of acute kidney injury (AKI). Materials and Methods: The canine AKI model was developed by i.v. injection of cisplatin and gentamycin into 14 male beagle dogs. cUCB-MSCs were administered into the renal corticomedullary junction following AKI induction. Survival time, clinical signs, blood analysis and histological parameters were analyzed. Results: The group treated with AKI plus cUCB-MSCs had decreased blood urea nitrogen and creatinine levels, and showed an extended lifespan and improved histological manifestations. MSCs were detected around the tubules of these kidneys at the histological level. Conclusion: Taken together, our findings suggest that cUCB-MSCs could be an alternative therapeutic agent for canine AKI.

AB - Background/Aim: The kidney excretes waste materials and regulates important metabolic functions, and renal disorders constitute a significant medical problem and can result in fatalities. In the present study, mesenchymal stem cells derived from canine umbilical cord blood (cUCB-MSCs) were isolated and evaluated for their ability to improve renal function in a canine model of acute kidney injury (AKI). Materials and Methods: The canine AKI model was developed by i.v. injection of cisplatin and gentamycin into 14 male beagle dogs. cUCB-MSCs were administered into the renal corticomedullary junction following AKI induction. Survival time, clinical signs, blood analysis and histological parameters were analyzed. Results: The group treated with AKI plus cUCB-MSCs had decreased blood urea nitrogen and creatinine levels, and showed an extended lifespan and improved histological manifestations. MSCs were detected around the tubules of these kidneys at the histological level. Conclusion: Taken together, our findings suggest that cUCB-MSCs could be an alternative therapeutic agent for canine AKI.

KW - Acute kidney injury

KW - Canine umbilical cord blood-mesenchymal stem cells

KW - Dog

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Mesenchymal stem cells contribute to improvement of renal function in a canine kidney injury model

Abstract

Keywords

UN SDGs

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