Metabolic profiling and method validation of marker compounds from saposhnikoviae radix and peucedani japonici radix

Bo Ram Choi; Dahye Yoon; Geum Soog Kim; Kyung Sook Han; Doo Jin Choi; Young Seob Lee; Do Yoon Hyun; Dae Young Lee

doi:10.3839/jabc.2020.051

Metabolic profiling and method validation of marker compounds from saposhnikoviae radix and peucedani japonici radix

Bo Ram Choi, Dahye Yoon, Geum Soog Kim, Kyung Sook Han, Doo Jin Choi, Young Seob Lee, Do Yoon Hyun, Dae Young Lee

School of Life Science and Biotechnology

Rural Development Administration

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Saposhnikoviae Radix (SR) and Peucedani Japonici Radix (PR) have been used as the main traditional herbal medicines in Korea, China and Japan. In this study, ultra-performance liquid chromatography coupled to quadrupole time of flight mass spectrometry (UPLC-QTOF/MS)-based metabolomics was applied to evaluate the quality of SR and PR using the marker compounds. In the S-plot of SR and PR, 5-O-methylvisammioside and peucedanol were selected as a marker compound for SR and PR, respectively. Also, an UPLC method was established and well validated for marker compounds of SR and PR. These results suggested that the established analysis method could be used one of the good methods for the classification and quality assessment of SR and PR.

Original language	English
Pages (from-to)	393-399
Number of pages	7
Journal	Journal of Applied Biological Chemistry
Volume	63
Issue number	4
DOIs	https://doi.org/10.3839/jabc.2020.051
State	Published - 2020

Keywords

Metabolic profiling
Method validation 5-O-methylvisammioside
Peucedani Japonici Radix
Peucedanol
Saposhnikoviae Radix

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title = "Metabolic profiling and method validation of marker compounds from saposhnikoviae radix and peucedani japonici radix",

abstract = "Saposhnikoviae Radix (SR) and Peucedani Japonici Radix (PR) have been used as the main traditional herbal medicines in Korea, China and Japan. In this study, ultra-performance liquid chromatography coupled to quadrupole time of flight mass spectrometry (UPLC-QTOF/MS)-based metabolomics was applied to evaluate the quality of SR and PR using the marker compounds. In the S-plot of SR and PR, 5-O-methylvisammioside and peucedanol were selected as a marker compound for SR and PR, respectively. Also, an UPLC method was established and well validated for marker compounds of SR and PR. These results suggested that the established analysis method could be used one of the good methods for the classification and quality assessment of SR and PR.",

keywords = "Metabolic profiling, Method validation 5-O-methylvisammioside, Peucedani Japonici Radix, Peucedanol, Saposhnikoviae Radix",

author = "Choi, {Bo Ram} and Dahye Yoon and Kim, {Geum Soog} and Han, {Kyung Sook} and Choi, {Doo Jin} and Lee, {Young Seob} and Hyun, {Do Yoon} and Lee, {Dae Young}",

note = "Publisher Copyright: {\textcopyright} The Korean Society for Applied Biological Chemistry 2020.",

year = "2020",

doi = "10.3839/jabc.2020.051",

language = "English",

volume = "63",

pages = "393--399",

journal = "Journal of Applied Biological Chemistry",

issn = "1976-0442",

publisher = "The Korean Society for Applied Biological Chemistry",

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TY - JOUR

T1 - Metabolic profiling and method validation of marker compounds from saposhnikoviae radix and peucedani japonici radix

AU - Choi, Bo Ram

AU - Yoon, Dahye

AU - Kim, Geum Soog

AU - Han, Kyung Sook

AU - Choi, Doo Jin

AU - Lee, Young Seob

AU - Hyun, Do Yoon

AU - Lee, Dae Young

N1 - Publisher Copyright: © The Korean Society for Applied Biological Chemistry 2020.

PY - 2020

Y1 - 2020

N2 - Saposhnikoviae Radix (SR) and Peucedani Japonici Radix (PR) have been used as the main traditional herbal medicines in Korea, China and Japan. In this study, ultra-performance liquid chromatography coupled to quadrupole time of flight mass spectrometry (UPLC-QTOF/MS)-based metabolomics was applied to evaluate the quality of SR and PR using the marker compounds. In the S-plot of SR and PR, 5-O-methylvisammioside and peucedanol were selected as a marker compound for SR and PR, respectively. Also, an UPLC method was established and well validated for marker compounds of SR and PR. These results suggested that the established analysis method could be used one of the good methods for the classification and quality assessment of SR and PR.

AB - Saposhnikoviae Radix (SR) and Peucedani Japonici Radix (PR) have been used as the main traditional herbal medicines in Korea, China and Japan. In this study, ultra-performance liquid chromatography coupled to quadrupole time of flight mass spectrometry (UPLC-QTOF/MS)-based metabolomics was applied to evaluate the quality of SR and PR using the marker compounds. In the S-plot of SR and PR, 5-O-methylvisammioside and peucedanol were selected as a marker compound for SR and PR, respectively. Also, an UPLC method was established and well validated for marker compounds of SR and PR. These results suggested that the established analysis method could be used one of the good methods for the classification and quality assessment of SR and PR.

KW - Metabolic profiling

KW - Method validation 5-O-methylvisammioside

KW - Peucedani Japonici Radix

KW - Peucedanol

KW - Saposhnikoviae Radix

UR - http://www.scopus.com/inward/record.url?scp=85098859614&partnerID=8YFLogxK

U2 - 10.3839/jabc.2020.051

DO - 10.3839/jabc.2020.051

M3 - Article

AN - SCOPUS:85098859614

SN - 1976-0442

VL - 63

SP - 393

EP - 399

JO - Journal of Applied Biological Chemistry

JF - Journal of Applied Biological Chemistry

IS - 4

ER -

Metabolic profiling and method validation of marker compounds from saposhnikoviae radix and peucedani japonici radix

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