TY - JOUR
T1 - Metabolism-mediated induction of zinc tolerance in Brassica rapa by Burkholderia cepacia CS2-1
AU - Kang, Sang Mo
AU - Shahzad, Raheem
AU - Bilal, Saqib
AU - Khan, Abdul Latif
AU - You, Young Hyun
AU - Lee, Won Hee
AU - Ryu, Hee La
AU - Lee, Ko Eun
AU - Lee, In Jung
N1 - Publisher Copyright:
© 2017, The Microbiological Society of Korea and Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2017/12/1
Y1 - 2017/12/1
N2 - Brassica rapa (Chinese cabbage) is an essential component of traditional Korean food. However, the crop is often subject to zinc (Zn+) toxicity from contaminated irrigation water, which, as a result, compromises plant growth and production, as well as the health of human consumers. The present study investigated the bioaccumulation of Zn+ by Burkholderia cepacia CS2-1 and its effect on the heavy metal tolerance of Chinese cabbage. Strain CS2-1 was identified and characterized on the basis of 16S rRNA sequences and phylogenetic analysis. The strain actively produced indole-3-acetic acid (3.08 ± 0.21 μg/ml) and was also able to produce siderophore, solubilize minerals, and tolerate various concentrations of Zn+. The heavy metal tolerance of B. rapa plants was enhanced by CS2-1 inoculation, as indicated by growth attributes, Zn+ uptake, amino acid synthesis, antioxidant levels, and endogenous hormone (ABA and SA) synthesis. Without inoculation, the application of Zn+ negatively affected the growth and physiology of B. rapa plants. However, CS2-1 inoculation improved plant growth, lowered Zn+ uptake, altered both amino acid regulation and levels of flavonoids and phenolics, and significantly decreased levels of superoxide dismutase, endogenous abscisic acid, and salicylic acid. These findings indicate that B. cepacia CS2-1 is suitable for bioremediation against Zn+-induced oxidative stress.
AB - Brassica rapa (Chinese cabbage) is an essential component of traditional Korean food. However, the crop is often subject to zinc (Zn+) toxicity from contaminated irrigation water, which, as a result, compromises plant growth and production, as well as the health of human consumers. The present study investigated the bioaccumulation of Zn+ by Burkholderia cepacia CS2-1 and its effect on the heavy metal tolerance of Chinese cabbage. Strain CS2-1 was identified and characterized on the basis of 16S rRNA sequences and phylogenetic analysis. The strain actively produced indole-3-acetic acid (3.08 ± 0.21 μg/ml) and was also able to produce siderophore, solubilize minerals, and tolerate various concentrations of Zn+. The heavy metal tolerance of B. rapa plants was enhanced by CS2-1 inoculation, as indicated by growth attributes, Zn+ uptake, amino acid synthesis, antioxidant levels, and endogenous hormone (ABA and SA) synthesis. Without inoculation, the application of Zn+ negatively affected the growth and physiology of B. rapa plants. However, CS2-1 inoculation improved plant growth, lowered Zn+ uptake, altered both amino acid regulation and levels of flavonoids and phenolics, and significantly decreased levels of superoxide dismutase, endogenous abscisic acid, and salicylic acid. These findings indicate that B. cepacia CS2-1 is suitable for bioremediation against Zn+-induced oxidative stress.
KW - abscisic acid
KW - amino acid
KW - antioxidant
KW - plant growth-promoting rhizobacterium
KW - salicylic acid
KW - zinc toxicity
UR - http://www.scopus.com/inward/record.url?scp=85037337776&partnerID=8YFLogxK
U2 - 10.1007/s12275-017-7305-7
DO - 10.1007/s12275-017-7305-7
M3 - Article
C2 - 29214486
AN - SCOPUS:85037337776
SN - 1225-8873
VL - 55
SP - 955
EP - 965
JO - Journal of Microbiology
JF - Journal of Microbiology
IS - 12
ER -
