TY - JOUR
T1 - Metabotropic glutamate receptors inhibit GABA release in rat histamine neurons
AU - Jang, Il Sung
PY - 2014/9/5
Y1 - 2014/9/5
N2 - The modulation of GABAergic transmission by metabotropic glutamate (mGlu) receptors was examined in histaminergic neurons using a conventional whole-cell patch clamp technique. DHPG, a selective group I mGlu receptor agonist, had no effect on GABAergic inhibitory postsynaptic currents (IPSCs). However, DCG-IV (1. μm{cyrillic}) and L-AP4 (1. μm{cyrillic}), selective group II or III mGlu receptor agonists, respectively, decreased the amplitude of GABAergic IPSCs and simultaneously increased the paired-pulse ratio. The inhibitory effect of DCG-IV was completely blocked by 200. nM LY341495, a group II and III mGlu receptor antagonist, and the inhibitory effect of L-AP4 was completely blocked by 10. μm{cyrillic} LY341495. These results suggest that multiple mGlu receptors are involved in regulating the excitability of histaminergic neurons.
AB - The modulation of GABAergic transmission by metabotropic glutamate (mGlu) receptors was examined in histaminergic neurons using a conventional whole-cell patch clamp technique. DHPG, a selective group I mGlu receptor agonist, had no effect on GABAergic inhibitory postsynaptic currents (IPSCs). However, DCG-IV (1. μm{cyrillic}) and L-AP4 (1. μm{cyrillic}), selective group II or III mGlu receptor agonists, respectively, decreased the amplitude of GABAergic IPSCs and simultaneously increased the paired-pulse ratio. The inhibitory effect of DCG-IV was completely blocked by 200. nM LY341495, a group II and III mGlu receptor antagonist, and the inhibitory effect of L-AP4 was completely blocked by 10. μm{cyrillic} LY341495. These results suggest that multiple mGlu receptors are involved in regulating the excitability of histaminergic neurons.
KW - GABAergic IPSCs
KW - Metabotropic glutamate receptors
KW - Patch clamp
KW - Sleep-wakefulness
KW - Tuberomammillary nucleus
UR - http://www.scopus.com/inward/record.url?scp=84905276890&partnerID=8YFLogxK
U2 - 10.1016/j.neulet.2014.07.022
DO - 10.1016/j.neulet.2014.07.022
M3 - Article
C2 - 25062585
AN - SCOPUS:84905276890
SN - 0304-3940
VL - 579
SP - 106
EP - 109
JO - Neuroscience Letters
JF - Neuroscience Letters
ER -