Microalgal photosynthetic activity measurement system for rapid toxicity assessment

Chul Woong Cho; Thi Phuong Thuy Pham; You Chul Jeon; Jiho Min; Ho Young Jung; Dae Sung Lee; Yeoung Sang Yun

doi:10.1007/s10646-008-0197-x

Microalgal photosynthetic activity measurement system for rapid toxicity assessment

Chul Woong Cho, Thi Phuong Thuy Pham, You Chul Jeon, Jiho Min, Ho Young Jung, Dae Sung Lee, Yeoung Sang Yun

Department of Environmental Engineering

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

A systematic biosensor is constructed for the estimation of toxic compounds based on photosynthetic activity measurement in Selenastrum capricornutum cells. The photosynthetic response was evaluated as a function of light intensity, cell concentration and initial dissolved oxygen. The inhibitory effect of some toxicants (1-butyl-3-methylimidazolium tetrafluoroborate, methanol) on dissolved oxygen production was also determined. In all cases, a toxic response was detected (i.e. a dose-related inhibition of photosynthetic activity was observed). For the present system, a time of only 2 h was needed to predict EC50 values as compared to 96 h for a conventional algal assay based on algal growth rate. Thus, the developed biosensor was proved to be useful as a rapid and simple test method in environmental toxicity assessment.

Original language	English
Pages (from-to)	455-463
Number of pages	9
Journal	Ecotoxicology
Volume	17
Issue number	6
DOIs	https://doi.org/10.1007/s10646-008-0197-x
State	Published - Aug 2008

Keywords

Biosensor
Oxygen evolution rate
Photosynthetic activity
Rapid toxicity test
Selenastrum capricornutum

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10.1007/s10646-008-0197-x

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title = "Microalgal photosynthetic activity measurement system for rapid toxicity assessment",

abstract = "A systematic biosensor is constructed for the estimation of toxic compounds based on photosynthetic activity measurement in Selenastrum capricornutum cells. The photosynthetic response was evaluated as a function of light intensity, cell concentration and initial dissolved oxygen. The inhibitory effect of some toxicants (1-butyl-3-methylimidazolium tetrafluoroborate, methanol) on dissolved oxygen production was also determined. In all cases, a toxic response was detected (i.e. a dose-related inhibition of photosynthetic activity was observed). For the present system, a time of only 2 h was needed to predict EC50 values as compared to 96 h for a conventional algal assay based on algal growth rate. Thus, the developed biosensor was proved to be useful as a rapid and simple test method in environmental toxicity assessment.",

keywords = "Biosensor, Oxygen evolution rate, Photosynthetic activity, Rapid toxicity test, Selenastrum capricornutum",

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T1 - Microalgal photosynthetic activity measurement system for rapid toxicity assessment

AU - Cho, Chul Woong

AU - Pham, Thi Phuong Thuy

AU - Jeon, You Chul

AU - Min, Jiho

AU - Jung, Ho Young

AU - Lee, Dae Sung

AU - Yun, Yeoung Sang

PY - 2008/8

Y1 - 2008/8

N2 - A systematic biosensor is constructed for the estimation of toxic compounds based on photosynthetic activity measurement in Selenastrum capricornutum cells. The photosynthetic response was evaluated as a function of light intensity, cell concentration and initial dissolved oxygen. The inhibitory effect of some toxicants (1-butyl-3-methylimidazolium tetrafluoroborate, methanol) on dissolved oxygen production was also determined. In all cases, a toxic response was detected (i.e. a dose-related inhibition of photosynthetic activity was observed). For the present system, a time of only 2 h was needed to predict EC50 values as compared to 96 h for a conventional algal assay based on algal growth rate. Thus, the developed biosensor was proved to be useful as a rapid and simple test method in environmental toxicity assessment.

AB - A systematic biosensor is constructed for the estimation of toxic compounds based on photosynthetic activity measurement in Selenastrum capricornutum cells. The photosynthetic response was evaluated as a function of light intensity, cell concentration and initial dissolved oxygen. The inhibitory effect of some toxicants (1-butyl-3-methylimidazolium tetrafluoroborate, methanol) on dissolved oxygen production was also determined. In all cases, a toxic response was detected (i.e. a dose-related inhibition of photosynthetic activity was observed). For the present system, a time of only 2 h was needed to predict EC50 values as compared to 96 h for a conventional algal assay based on algal growth rate. Thus, the developed biosensor was proved to be useful as a rapid and simple test method in environmental toxicity assessment.

KW - Biosensor

KW - Oxygen evolution rate

KW - Photosynthetic activity

KW - Rapid toxicity test

KW - Selenastrum capricornutum

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JO - Ecotoxicology

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ER -

Microalgal photosynthetic activity measurement system for rapid toxicity assessment

Abstract

Keywords

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