Microarray analysis of differentially expressed genes between cysts and trophozoites of acanthamoeba castellanii

Eun Kyung Moon, Ying Hua Xuan, Dong Il Chung, Yeonchul Hong, Hyun Hee Kong

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Acanthamoeba infection is difficult to treat because of the resistance property of Acanthamoeba cyst against the host immune system, diverse antibiotics, and therapeutic agents. To identify encystation mediating factors of Acanthamoeba, we compared the transcription profile between cysts and trophozoites using microarray analysis. The DNA chip was composed of 12,544 genes based on expressed sequence tag (EST) from an Acanthamoeba ESTs database (DB) constructed in our laboratory, genetic information of Acanthamoeba from TBest DB, and all of Acanthamoeba related genes registered in the NCBI. Microarray analysis indicated that 701 genes showed higher expression than 2 folds in cysts than in trophozoites, and 859 genes were less expressed in cysts than in trophozoites. The results of real-time PCR analysis of randomly selected 9 genes of which expression was increased during cyst formation were coincided well with the microarray results. Eukaryotic orthologous groups (KOG) analysis showed an increment in T article (signal transduction mechanisms) and O article (posttranslational modification, protein turnover, and chaperones) whereas significant decrement of C article (energy production and conversion) during cyst formation. Especially, cystein proteinases showed high expression changes (282 folds) with significant increases in real-time PCR, suggesting a pivotal role of this proteinase in the cyst formation of Acanthamoeba. The present study provides important clues for the identification and characterization of encystation mediating factors of Acanthamoeba.

Original languageEnglish
Pages (from-to)341-347
Number of pages7
JournalKorean Journal of Parasitology
Volume49
Issue number4
DOIs
StatePublished - Dec 2011

Keywords

  • Acanthamoeba
  • Cystein proteinase
  • Encystation
  • Microarray

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