Microfluidic Encapsulation of Vorinostat in Calcium Alginate Microparticles: Prolonged Release and Enhanced in vitro Cytotoxicity

In this research, the vorinostat-loaded alginate microparticles were created and their characterization was performed using scanning electron microscopy (SEM), energy-dispersive X-ray mapping (EDX-mapping), and transmission electron microscopy (TEM). Differential scanning calorimetry (DSC) was used to assess the drug state and thermal behavior of vorinostat within the microparticles. The encapsulation efficiency and loading efficiency were determined, and the solubility of vorinostat in the microparticles was investigated. In vitro drug release studies demonstrated sustained release of vorinostat from the microparticles. The cytotoxicity of vorinostat-loaded alginate microparticles was evaluated using HepG2, MCF-7, and SK-LU-1 cell lines. The results indicated significant inhibition of cell growth, and the IC₅₀ values were estimated. Overall, this study successfully demonstrated the fabrication of vorinostat-loaded alginate microparticles using a microfluidic approach, suggesting a simple and effective delivery system for vorinostat with improved physicochemical and pharmacological properties.