TY - JOUR
T1 - Micropositioning and microscopic observation of individual picoliter-sized containers within SU-8 microchannels
AU - Jenke, Martin G.
AU - Schreiter, Christoph
AU - Kim, Gyu Man
AU - Vogel, Horst
AU - Brugger, Jürgen
PY - 2007/4
Y1 - 2007/4
N2 - We describe the fabrication and application of a bioanalytical chip, made of SU-8 photoresist, comprising integrated, high aspect-ratio microfluidic channels, suitable to manipulate and investigate vesicles, cell fragments and biological cells. A central micrometer-sized aperture allows electrical particle counting and planar membrane experiments, microfluids allow (sub)micrometer-sized objects to be transported and addressed with different chemicals. Here we show how lipid vesicles are positioned with micrometer precision within the micro-channels by means of pressure and electrophoretic movement. Our approach is suited for controlling and investigating (bio)chemical synthesis and cellular signalling processes in ultrasmall individual vesicles by electro-optical techniques.
AB - We describe the fabrication and application of a bioanalytical chip, made of SU-8 photoresist, comprising integrated, high aspect-ratio microfluidic channels, suitable to manipulate and investigate vesicles, cell fragments and biological cells. A central micrometer-sized aperture allows electrical particle counting and planar membrane experiments, microfluids allow (sub)micrometer-sized objects to be transported and addressed with different chemicals. Here we show how lipid vesicles are positioned with micrometer precision within the micro-channels by means of pressure and electrophoretic movement. Our approach is suited for controlling and investigating (bio)chemical synthesis and cellular signalling processes in ultrasmall individual vesicles by electro-optical techniques.
KW - Electrophoretic micromanipulation
KW - Microfluidics
KW - Rapid prototyping
KW - SU-8
UR - http://www.scopus.com/inward/record.url?scp=33847141438&partnerID=8YFLogxK
U2 - 10.1007/s10404-006-0119-2
DO - 10.1007/s10404-006-0119-2
M3 - Article
AN - SCOPUS:33847141438
SN - 1613-4982
VL - 3
SP - 189
EP - 194
JO - Microfluidics and Nanofluidics
JF - Microfluidics and Nanofluidics
IS - 2
ER -