Micropositioning and microscopic observation of individual picoliter-sized containers within SU-8 microchannels

Martin G. Jenke; Christoph Schreiter; Gyu Man Kim; Horst Vogel; Jürgen Brugger

doi:10.1007/s10404-006-0119-2

Micropositioning and microscopic observation of individual picoliter-sized containers within SU-8 microchannels

Martin G. Jenke
, Christoph Schreiter
, Gyu Man Kim
, Horst Vogel
, Jürgen Brugger

School of Mechanical Engineering

Swiss Federal Institute of Technology Lausanne

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

We describe the fabrication and application of a bioanalytical chip, made of SU-8 photoresist, comprising integrated, high aspect-ratio microfluidic channels, suitable to manipulate and investigate vesicles, cell fragments and biological cells. A central micrometer-sized aperture allows electrical particle counting and planar membrane experiments, microfluids allow (sub)micrometer-sized objects to be transported and addressed with different chemicals. Here we show how lipid vesicles are positioned with micrometer precision within the micro-channels by means of pressure and electrophoretic movement. Our approach is suited for controlling and investigating (bio)chemical synthesis and cellular signalling processes in ultrasmall individual vesicles by electro-optical techniques.

Original language	English
Pages (from-to)	189-194
Number of pages	6
Journal	Microfluidics and Nanofluidics
Volume	3
Issue number	2
DOIs	https://doi.org/10.1007/s10404-006-0119-2
State	Published - Apr 2007

Keywords

Electrophoretic micromanipulation
Microfluidics
Rapid prototyping
SU-8

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10.1007/s10404-006-0119-2

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title = "Micropositioning and microscopic observation of individual picoliter-sized containers within SU-8 microchannels",

abstract = "We describe the fabrication and application of a bioanalytical chip, made of SU-8 photoresist, comprising integrated, high aspect-ratio microfluidic channels, suitable to manipulate and investigate vesicles, cell fragments and biological cells. A central micrometer-sized aperture allows electrical particle counting and planar membrane experiments, microfluids allow (sub)micrometer-sized objects to be transported and addressed with different chemicals. Here we show how lipid vesicles are positioned with micrometer precision within the micro-channels by means of pressure and electrophoretic movement. Our approach is suited for controlling and investigating (bio)chemical synthesis and cellular signalling processes in ultrasmall individual vesicles by electro-optical techniques.",

keywords = "Electrophoretic micromanipulation, Microfluidics, Rapid prototyping, SU-8",

author = "Jenke, \{Martin G.\} and Christoph Schreiter and Kim, \{Gyu Man\} and Horst Vogel and J{\"u}rgen Brugger",

year = "2007",

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doi = "10.1007/s10404-006-0119-2",

language = "English",

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AU - Jenke, Martin G.

AU - Schreiter, Christoph

AU - Kim, Gyu Man

AU - Vogel, Horst

AU - Brugger, Jürgen

PY - 2007/4

Y1 - 2007/4

N2 - We describe the fabrication and application of a bioanalytical chip, made of SU-8 photoresist, comprising integrated, high aspect-ratio microfluidic channels, suitable to manipulate and investigate vesicles, cell fragments and biological cells. A central micrometer-sized aperture allows electrical particle counting and planar membrane experiments, microfluids allow (sub)micrometer-sized objects to be transported and addressed with different chemicals. Here we show how lipid vesicles are positioned with micrometer precision within the micro-channels by means of pressure and electrophoretic movement. Our approach is suited for controlling and investigating (bio)chemical synthesis and cellular signalling processes in ultrasmall individual vesicles by electro-optical techniques.

AB - We describe the fabrication and application of a bioanalytical chip, made of SU-8 photoresist, comprising integrated, high aspect-ratio microfluidic channels, suitable to manipulate and investigate vesicles, cell fragments and biological cells. A central micrometer-sized aperture allows electrical particle counting and planar membrane experiments, microfluids allow (sub)micrometer-sized objects to be transported and addressed with different chemicals. Here we show how lipid vesicles are positioned with micrometer precision within the micro-channels by means of pressure and electrophoretic movement. Our approach is suited for controlling and investigating (bio)chemical synthesis and cellular signalling processes in ultrasmall individual vesicles by electro-optical techniques.

KW - Electrophoretic micromanipulation

KW - Microfluidics

KW - Rapid prototyping

KW - SU-8

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M3 - Article

AN - SCOPUS:33847141438

SN - 1613-4982

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JO - Microfluidics and Nanofluidics

JF - Microfluidics and Nanofluidics

IS - 2

ER -

Micropositioning and microscopic observation of individual picoliter-sized containers within SU-8 microchannels

Abstract

Keywords

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