TY - JOUR
T1 - Modeling and optimization of photosynthetic hydrogen gas production by green alga Chlamydomonas reinhardtii in sulfur-deprived circumstance
AU - Jo, Ji Hye
AU - Lee, Dae Sung
AU - Park, Jong Moon
PY - 2006/3
Y1 - 2006/3
N2 - Biological hydrogen production by the green alga Chlamydomonas reinhardtii under sulfur-deprived conditions has attracted great interest due to the fundamental and practical importance of the process. The photosynthetic hydrogen production rate is dependent on various factors such as strain type, nutrient composition, temperature, pH, and light intensity. In this study, physicochemical factors affecting biological hydrogen production by C. reinhardtii were evaluated with response surface methodology (RSM). First, the maximum specific growth rate of the alga associated with simultaneous changes of ammonium, phosphate, and sulfate concentrations in the culture medium were investigated. The optimum conditions were determined as NH4 + 8.00 mM, PO43- 1.11 mM, and SO 42- 0.79 mM in Tris-acetate-phosphate (TAP) medium. The maximum specific growth rate with the optimum nutrient concentrations was 0.0373 h-1. Then, the hydrogen production rate of C. reinhardtii under sulfur-deprivation conditions was investigated by simultaneously changing two nutrient concentrations and pH in the medium. The maximum hydrogen production was 2.152 mL of H2 for a 10-mL culture of alga with density of 6 × 106 cells mL-1 for 96 h under conditions of NH4+ 9.20 mM, PO43- 2.09 mM, and pH 7.00. The obtained hydrogen production rate was approximately 1.55 times higher than that with the typical TAP medium under sulfur deficiency.
AB - Biological hydrogen production by the green alga Chlamydomonas reinhardtii under sulfur-deprived conditions has attracted great interest due to the fundamental and practical importance of the process. The photosynthetic hydrogen production rate is dependent on various factors such as strain type, nutrient composition, temperature, pH, and light intensity. In this study, physicochemical factors affecting biological hydrogen production by C. reinhardtii were evaluated with response surface methodology (RSM). First, the maximum specific growth rate of the alga associated with simultaneous changes of ammonium, phosphate, and sulfate concentrations in the culture medium were investigated. The optimum conditions were determined as NH4 + 8.00 mM, PO43- 1.11 mM, and SO 42- 0.79 mM in Tris-acetate-phosphate (TAP) medium. The maximum specific growth rate with the optimum nutrient concentrations was 0.0373 h-1. Then, the hydrogen production rate of C. reinhardtii under sulfur-deprivation conditions was investigated by simultaneously changing two nutrient concentrations and pH in the medium. The maximum hydrogen production was 2.152 mL of H2 for a 10-mL culture of alga with density of 6 × 106 cells mL-1 for 96 h under conditions of NH4+ 9.20 mM, PO43- 2.09 mM, and pH 7.00. The obtained hydrogen production rate was approximately 1.55 times higher than that with the typical TAP medium under sulfur deficiency.
UR - http://www.scopus.com/inward/record.url?scp=33646019836&partnerID=8YFLogxK
U2 - 10.1021/bp050258z
DO - 10.1021/bp050258z
M3 - Article
C2 - 16599558
AN - SCOPUS:33646019836
SN - 8756-7938
VL - 22
SP - 431
EP - 437
JO - Biotechnology Progress
JF - Biotechnology Progress
IS - 2
ER -