Molecular and biological characterization of an isolate of cucumber mosaic virus from Glycine soja by generating its infectious full-genome cDNA clones

Mi Sa Vo Phan; Jang Kyun Seo; Hong Soo Choi; Su Heon Lee; Kook Hyung Kim

doi:10.5423/PPJ.OA.02.2014.0014

Molecular and biological characterization of an isolate of cucumber mosaic virus from Glycine soja by generating its infectious full-genome cDNA clones

Mi Sa Vo Phan, Jang Kyun Seo, Hong Soo Choi, Su Heon Lee, Kook Hyung Kim

Department of Plant Medicine

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Molecular and biological characteristics of an isolate of Cucumber mosaic virus (CMV) from Glycine soja (wild soybean), named as CMV-209, was examined in this study. Comparison of nucleotide sequences and phylogenetic analyses of CMV-209 with the other CMV strains revealed that CMV-209 belonged to CMV subgroup I. However, CMV-209 showed some genetic distance from the CMV strains assigned to subgroup IA or subgroup IB. Infectious full-genome cDNA clones of CMV-209 were generated under the control of the Cauliflower mosaic virus 35S promoter. Infectivity of the CMV-209 clones was evaluated in Nicotiana benthamiana and various legume species. Our assays revealed that CMV-209 could systemically infect Glycine soja (wild soybean) and Pisum sativum (pea) as well as N. benthamiana, but not the other legume species.

Original language	English
Pages (from-to)	159-167
Number of pages	9
Journal	Plant Pathology Journal
Volume	30
Issue number	2
DOIs	https://doi.org/10.5423/PPJ.OA.02.2014.0014
State	Published - 2014

Keywords

Cucumber mosaic virus
Infectious clones
Phylogenetic analyses
Sequence analysis
Wild soybean

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title = "Molecular and biological characterization of an isolate of cucumber mosaic virus from Glycine soja by generating its infectious full-genome cDNA clones",

abstract = "Molecular and biological characteristics of an isolate of Cucumber mosaic virus (CMV) from Glycine soja (wild soybean), named as CMV-209, was examined in this study. Comparison of nucleotide sequences and phylogenetic analyses of CMV-209 with the other CMV strains revealed that CMV-209 belonged to CMV subgroup I. However, CMV-209 showed some genetic distance from the CMV strains assigned to subgroup IA or subgroup IB. Infectious full-genome cDNA clones of CMV-209 were generated under the control of the Cauliflower mosaic virus 35S promoter. Infectivity of the CMV-209 clones was evaluated in Nicotiana benthamiana and various legume species. Our assays revealed that CMV-209 could systemically infect Glycine soja (wild soybean) and Pisum sativum (pea) as well as N. benthamiana, but not the other legume species.",

keywords = "Cucumber mosaic virus, Infectious clones, Phylogenetic analyses, Sequence analysis, Wild soybean",

author = "Phan, {Mi Sa Vo} and Seo, {Jang Kyun} and Choi, {Hong Soo} and Lee, {Su Heon} and Kim, {Kook Hyung}",

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T1 - Molecular and biological characterization of an isolate of cucumber mosaic virus from Glycine soja by generating its infectious full-genome cDNA clones

AU - Phan, Mi Sa Vo

AU - Seo, Jang Kyun

AU - Choi, Hong Soo

AU - Lee, Su Heon

AU - Kim, Kook Hyung

PY - 2014

Y1 - 2014

N2 - Molecular and biological characteristics of an isolate of Cucumber mosaic virus (CMV) from Glycine soja (wild soybean), named as CMV-209, was examined in this study. Comparison of nucleotide sequences and phylogenetic analyses of CMV-209 with the other CMV strains revealed that CMV-209 belonged to CMV subgroup I. However, CMV-209 showed some genetic distance from the CMV strains assigned to subgroup IA or subgroup IB. Infectious full-genome cDNA clones of CMV-209 were generated under the control of the Cauliflower mosaic virus 35S promoter. Infectivity of the CMV-209 clones was evaluated in Nicotiana benthamiana and various legume species. Our assays revealed that CMV-209 could systemically infect Glycine soja (wild soybean) and Pisum sativum (pea) as well as N. benthamiana, but not the other legume species.

AB - Molecular and biological characteristics of an isolate of Cucumber mosaic virus (CMV) from Glycine soja (wild soybean), named as CMV-209, was examined in this study. Comparison of nucleotide sequences and phylogenetic analyses of CMV-209 with the other CMV strains revealed that CMV-209 belonged to CMV subgroup I. However, CMV-209 showed some genetic distance from the CMV strains assigned to subgroup IA or subgroup IB. Infectious full-genome cDNA clones of CMV-209 were generated under the control of the Cauliflower mosaic virus 35S promoter. Infectivity of the CMV-209 clones was evaluated in Nicotiana benthamiana and various legume species. Our assays revealed that CMV-209 could systemically infect Glycine soja (wild soybean) and Pisum sativum (pea) as well as N. benthamiana, but not the other legume species.

KW - Cucumber mosaic virus

KW - Infectious clones

KW - Phylogenetic analyses

KW - Sequence analysis

KW - Wild soybean

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JO - Plant Pathology Journal

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IS - 2

ER -

Molecular and biological characterization of an isolate of cucumber mosaic virus from Glycine soja by generating its infectious full-genome cDNA clones

Abstract

Keywords

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