Molecular and phylogenetic analysis of equine piroplasms in the Republic of Korea

Min Goo Seo; Sun Hee Yun; Seong Kyoon Choi; Gil Jae Cho; Yong Soo Park; Kwang Hyun Cho; Oh Deog Kwon; Dongmi Kwak

doi:10.1016/j.rvsc.2013.01.014

Molecular and phylogenetic analysis of equine piroplasms in the Republic of Korea

Min Goo Seo, Sun Hee Yun, Seong Kyoon Choi, Gil Jae Cho, Yong Soo Park, Kwang Hyun Cho, Oh Deog Kwon, Dongmi Kwak

Department of Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

This study was conducted to screen out horses infected with piroplasms using PCR and to assess the phylogenetic variations of the piroplasm isolates. From 2007 to 2010, a total of 224 blood samples of horses were collected from three provinces of Korea and analyzed by PCR using primers specific to the 18S rRNA of piroplasms. Out of 224 samples analyzed, only two (0.9%) horses were found positive for Theileria equi. Sequencing of the complete 18S rRNA of T. equi from the two horses (GG-7 and GG-14) whose information was submitted to the GenBank (accession nos. HM229407 and HM229408, respectively) showed 100% identity. Alignment of the complete sequences of T. equi 18S rRNA with the GenBank databases of T. equi showed a high degree of homology (98.6-99.8%). The phylogenetic analysis showed T. equi GG-7 and GG-14 clustered together with T. equi isolates from Spain, Sudan, Jordan and South Africa, indicating the possibility of a close epidemiological link among these isolates.

Original language	English
Pages (from-to)	579-583
Number of pages	5
Journal	Research in Veterinary Science
Volume	94
Issue number	3
DOIs	https://doi.org/10.1016/j.rvsc.2013.01.014
State	Published - Jun 2013

Keywords

Horse blood
Korea
PCR
Phylogram
Piroplasm
Theileria equi

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10.1016/j.rvsc.2013.01.014

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title = "Molecular and phylogenetic analysis of equine piroplasms in the Republic of Korea",

abstract = "This study was conducted to screen out horses infected with piroplasms using PCR and to assess the phylogenetic variations of the piroplasm isolates. From 2007 to 2010, a total of 224 blood samples of horses were collected from three provinces of Korea and analyzed by PCR using primers specific to the 18S rRNA of piroplasms. Out of 224 samples analyzed, only two (0.9%) horses were found positive for Theileria equi. Sequencing of the complete 18S rRNA of T. equi from the two horses (GG-7 and GG-14) whose information was submitted to the GenBank (accession nos. HM229407 and HM229408, respectively) showed 100% identity. Alignment of the complete sequences of T. equi 18S rRNA with the GenBank databases of T. equi showed a high degree of homology (98.6-99.8%). The phylogenetic analysis showed T. equi GG-7 and GG-14 clustered together with T. equi isolates from Spain, Sudan, Jordan and South Africa, indicating the possibility of a close epidemiological link among these isolates.",

keywords = "Horse blood, Korea, PCR, Phylogram, Piroplasm, Theileria equi",

author = "Seo, {Min Goo} and Yun, {Sun Hee} and Choi, {Seong Kyoon} and Cho, {Gil Jae} and Park, {Yong Soo} and Cho, {Kwang Hyun} and Kwon, {Oh Deog} and Dongmi Kwak",

year = "2013",

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language = "English",

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AU - Seo, Min Goo

AU - Yun, Sun Hee

AU - Choi, Seong Kyoon

AU - Cho, Gil Jae

AU - Park, Yong Soo

AU - Cho, Kwang Hyun

AU - Kwon, Oh Deog

AU - Kwak, Dongmi

PY - 2013/6

Y1 - 2013/6

N2 - This study was conducted to screen out horses infected with piroplasms using PCR and to assess the phylogenetic variations of the piroplasm isolates. From 2007 to 2010, a total of 224 blood samples of horses were collected from three provinces of Korea and analyzed by PCR using primers specific to the 18S rRNA of piroplasms. Out of 224 samples analyzed, only two (0.9%) horses were found positive for Theileria equi. Sequencing of the complete 18S rRNA of T. equi from the two horses (GG-7 and GG-14) whose information was submitted to the GenBank (accession nos. HM229407 and HM229408, respectively) showed 100% identity. Alignment of the complete sequences of T. equi 18S rRNA with the GenBank databases of T. equi showed a high degree of homology (98.6-99.8%). The phylogenetic analysis showed T. equi GG-7 and GG-14 clustered together with T. equi isolates from Spain, Sudan, Jordan and South Africa, indicating the possibility of a close epidemiological link among these isolates.

AB - This study was conducted to screen out horses infected with piroplasms using PCR and to assess the phylogenetic variations of the piroplasm isolates. From 2007 to 2010, a total of 224 blood samples of horses were collected from three provinces of Korea and analyzed by PCR using primers specific to the 18S rRNA of piroplasms. Out of 224 samples analyzed, only two (0.9%) horses were found positive for Theileria equi. Sequencing of the complete 18S rRNA of T. equi from the two horses (GG-7 and GG-14) whose information was submitted to the GenBank (accession nos. HM229407 and HM229408, respectively) showed 100% identity. Alignment of the complete sequences of T. equi 18S rRNA with the GenBank databases of T. equi showed a high degree of homology (98.6-99.8%). The phylogenetic analysis showed T. equi GG-7 and GG-14 clustered together with T. equi isolates from Spain, Sudan, Jordan and South Africa, indicating the possibility of a close epidemiological link among these isolates.

KW - Horse blood

KW - Korea

KW - PCR

KW - Phylogram

KW - Piroplasm

KW - Theileria equi

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Molecular and phylogenetic analysis of equine piroplasms in the Republic of Korea

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