TY - JOUR
T1 - Molecular and serological prevalence of Babesia bovis and Babesia bigemina in cattle from central region of Syria
AU - Terkawi, Mohamad Alaa
AU - Alhasan, Hend
AU - Huyen, Nguyen Xuan
AU - Sabagh, Amin
AU - Awier, Karam
AU - Cao, Shinuo
AU - Goo, Youn Kyoung
AU - Aboge, Gabriel
AU - Yokoyama, Naoaki
AU - Nishikawa, Yoshifumi
AU - Kalb-Allouz, Abdul Karim
AU - Tabbaa, Darem
AU - Igarashi, Ikuo
AU - Xuan, Xuenan
PY - 2012/6/8
Y1 - 2012/6/8
N2 - A total of 207 bovine blood samples were collected from clinically healthy cattle bred in central region of Syria and examined by Giemsa-stained blood smears, nested PCR, ELISA, and IFAT to determine the molecular and serological prevalence of Babesia bovis and B. bigemina. All samples were negative to Babesia spp. by microscopic examination of blood smears. On the other hand, the overall prevalence of B. bovis and B. bigemina was 9.18% and 15.46% by nPCR, 15.46% and 18.84% by ELISA, and 18.36% and 21.74% by IFAT, respectively. Mixed infections were detected in a total of 5 samples (2.4%) by nPCR, 16 (7.73%) by ELISA and 27 (13.04%) by IFAT. Statistically significant differences in the prevalence of the two infections were observed on the basis of age and location. These data provide valuable information regarding the occurrence and epidemiology of B. bovis and B. bigemina infections in Syrian cattle, which can be employed in developing rational strategies for disease control and management.
AB - A total of 207 bovine blood samples were collected from clinically healthy cattle bred in central region of Syria and examined by Giemsa-stained blood smears, nested PCR, ELISA, and IFAT to determine the molecular and serological prevalence of Babesia bovis and B. bigemina. All samples were negative to Babesia spp. by microscopic examination of blood smears. On the other hand, the overall prevalence of B. bovis and B. bigemina was 9.18% and 15.46% by nPCR, 15.46% and 18.84% by ELISA, and 18.36% and 21.74% by IFAT, respectively. Mixed infections were detected in a total of 5 samples (2.4%) by nPCR, 16 (7.73%) by ELISA and 27 (13.04%) by IFAT. Statistically significant differences in the prevalence of the two infections were observed on the basis of age and location. These data provide valuable information regarding the occurrence and epidemiology of B. bovis and B. bigemina infections in Syrian cattle, which can be employed in developing rational strategies for disease control and management.
KW - B. bigemina
KW - Babesia bovis
KW - Diagnosis
KW - ELISA
KW - IFAT
KW - NPCR
UR - http://www.scopus.com/inward/record.url?scp=84860699307&partnerID=8YFLogxK
U2 - 10.1016/j.vetpar.2011.12.038
DO - 10.1016/j.vetpar.2011.12.038
M3 - Article
C2 - 22265803
AN - SCOPUS:84860699307
SN - 0304-4017
VL - 187
SP - 307
EP - 311
JO - Veterinary Parasitology
JF - Veterinary Parasitology
IS - 1-2
ER -