Molecular cloning and functional expression of bovine brain GABA transaminase

Seong Gyu Jeon; Jae Hoon Bahn; Joong Sik Jang; Sang Ho Jang; Byung Ryong Lee; Kil Soo Lee; Jinseu Park; Tae Cheon Kang; Moo Ho Won; Hyong Bai Kim; Oh Shin Kwon; Sung Woo Cho; Soo Young Choi

Molecular cloning and functional expression of bovine brain GABA transaminase

Seong Gyu Jeon, Jae Hoon Bahn, Joong Sik Jang, Sang Ho Jang, Byung Ryong Lee, Kil Soo Lee, Jinseu Park, Tae Cheon Kang, Moo Ho Won, Hyong Bai Kim, Oh Shin Kwon, Sung Woo Cho, Soo Young Choi

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

We isolated a cDNA that encodes the bovine brain γ-aminobutyrate transaminase (GABA-T; EC 2.6.1.19) from the λgt 11 cDNA library, which showed a high degree of sequence similarity to the corresponding enzymes from various sources. Northern blot analysis revealed two differentially expressed GABA-T transcripts of approximately 2.0 and 6.0 kb in the bovine tissues. Southern blot analysis indicates that the two GABA-T transcripts are encoded in a greater-than 10-kb, single-copy gene. Bovine GABA-T cDNA was expressed in E. coli using the pGEX bacterial-expression vector system. The overexpressed GABA-T was enzymatically active after purification, and it had very similar kinetic parameters when compared with those of other mammalian GABA-Ts.

Original language	English
Pages (from-to)	91-96
Number of pages	6
Journal	Molecules and Cells
Volume	12
Issue number	1
State	Published - 31 Aug 2001

Keywords

cDNA Library
GABA-T
Northern Blot
Southern Hybridization

Cite this

@article{887ec2e0094f4eea807c6ff53839df10,

title = "Molecular cloning and functional expression of bovine brain GABA transaminase",

abstract = "We isolated a cDNA that encodes the bovine brain γ-aminobutyrate transaminase (GABA-T; EC 2.6.1.19) from the λgt 11 cDNA library, which showed a high degree of sequence similarity to the corresponding enzymes from various sources. Northern blot analysis revealed two differentially expressed GABA-T transcripts of approximately 2.0 and 6.0 kb in the bovine tissues. Southern blot analysis indicates that the two GABA-T transcripts are encoded in a greater-than 10-kb, single-copy gene. Bovine GABA-T cDNA was expressed in E. coli using the pGEX bacterial-expression vector system. The overexpressed GABA-T was enzymatically active after purification, and it had very similar kinetic parameters when compared with those of other mammalian GABA-Ts.",

keywords = "cDNA Library, GABA-T, Northern Blot, Southern Hybridization",

author = "Jeon, {Seong Gyu} and Bahn, {Jae Hoon} and Jang, {Joong Sik} and Jang, {Sang Ho} and Lee, {Byung Ryong} and Lee, {Kil Soo} and Jinseu Park and Kang, {Tae Cheon} and Won, {Moo Ho} and Kim, {Hyong Bai} and Kwon, {Oh Shin} and Cho, {Sung Woo} and Choi, {Soo Young}",

year = "2001",

month = aug,

day = "31",

language = "English",

volume = "12",

pages = "91--96",

journal = "Molecules and Cells",

issn = "1016-8478",

publisher = "Korean Society for Molecular and Cellular Biology",

number = "1",

}

TY - JOUR

T1 - Molecular cloning and functional expression of bovine brain GABA transaminase

AU - Jeon, Seong Gyu

AU - Bahn, Jae Hoon

AU - Jang, Joong Sik

AU - Jang, Sang Ho

AU - Lee, Byung Ryong

AU - Lee, Kil Soo

AU - Park, Jinseu

AU - Kang, Tae Cheon

AU - Won, Moo Ho

AU - Kim, Hyong Bai

AU - Kwon, Oh Shin

AU - Cho, Sung Woo

AU - Choi, Soo Young

PY - 2001/8/31

Y1 - 2001/8/31

N2 - We isolated a cDNA that encodes the bovine brain γ-aminobutyrate transaminase (GABA-T; EC 2.6.1.19) from the λgt 11 cDNA library, which showed a high degree of sequence similarity to the corresponding enzymes from various sources. Northern blot analysis revealed two differentially expressed GABA-T transcripts of approximately 2.0 and 6.0 kb in the bovine tissues. Southern blot analysis indicates that the two GABA-T transcripts are encoded in a greater-than 10-kb, single-copy gene. Bovine GABA-T cDNA was expressed in E. coli using the pGEX bacterial-expression vector system. The overexpressed GABA-T was enzymatically active after purification, and it had very similar kinetic parameters when compared with those of other mammalian GABA-Ts.

AB - We isolated a cDNA that encodes the bovine brain γ-aminobutyrate transaminase (GABA-T; EC 2.6.1.19) from the λgt 11 cDNA library, which showed a high degree of sequence similarity to the corresponding enzymes from various sources. Northern blot analysis revealed two differentially expressed GABA-T transcripts of approximately 2.0 and 6.0 kb in the bovine tissues. Southern blot analysis indicates that the two GABA-T transcripts are encoded in a greater-than 10-kb, single-copy gene. Bovine GABA-T cDNA was expressed in E. coli using the pGEX bacterial-expression vector system. The overexpressed GABA-T was enzymatically active after purification, and it had very similar kinetic parameters when compared with those of other mammalian GABA-Ts.

KW - cDNA Library

KW - GABA-T

KW - Northern Blot

KW - Southern Hybridization

UR - http://www.scopus.com/inward/record.url?scp=0035980038&partnerID=8YFLogxK

M3 - Article

C2 - 11561735

AN - SCOPUS:0035980038

SN - 1016-8478

VL - 12

SP - 91

EP - 96

JO - Molecules and Cells

JF - Molecules and Cells

IS - 1

ER -

Molecular cloning and functional expression of bovine brain GABA transaminase

Abstract

Keywords

Other files and links

Fingerprint

Cite this