Molecular cloning and functional expression of bovine brain GABA transaminase

Seong Gyu Jeon; Jae Hoon Bahn; Joong Sik Jang; Sang Ho Jang; Byung Ryong Lee; Kil Soo Lee; Jinseu Park; Tae Cheon Kang; Moo Ho Won; Hyong Bai Kim; Oh Shin Kwon; Sung Woo Cho; Soo Young Choi

doi:10.1016/s1016-8478(23)17066-x

Molecular cloning and functional expression of bovine brain GABA transaminase

Seong Gyu Jeon
, Jae Hoon Bahn
, Joong Sik Jang
, Sang Ho Jang
, Byung Ryong Lee
, Kil Soo Lee
, Jinseu Park
, Tae Cheon Kang
, Moo Ho Won
, Hyong Bai Kim
, Oh Shin Kwon
, Sung Woo Cho
, Soo Young Choi

Hallym University
Korea University
University of Ulsan

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

We isolated a cDNA that encodes the bovine brain γ-aminobutyrate transaminase (GABA-T; EC 2.6.1.19) from the λgt 11 cDNA library, which showed a high degree of sequence similarity to the corresponding enzymes from various sources. Northern blot analysis revealed two differentially expressed GABA-T transcripts of approximately 2.0 and 6.0 kb in the bovine tissues. Southern blot analysis indicates that the two GABA-T transcripts are encoded in a greater-than 10-kb, single-copy gene. Bovine GABA-T cDNA was expressed in E. coli using the pGEX bacterial-expression vector system. The overexpressed GABA-T was enzymatically active after purification, and it had very similar kinetic parameters when compared with those of other mammalian GABA-Ts.

Original language	English
Pages (from-to)	91-96
Number of pages	6
Journal	Molecules and Cells
Volume	12
Issue number	1
DOIs	https://doi.org/10.1016/s1016-8478(23)17066-x
State	Published - 31 Aug 2001

Keywords

GABA-T
Northern Blot
Southern Hybridization
cDNA Library

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10.1016/s1016-8478(23)17066-x

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title = "Molecular cloning and functional expression of bovine brain GABA transaminase",

abstract = "We isolated a cDNA that encodes the bovine brain γ-aminobutyrate transaminase (GABA-T; EC 2.6.1.19) from the λgt 11 cDNA library, which showed a high degree of sequence similarity to the corresponding enzymes from various sources. Northern blot analysis revealed two differentially expressed GABA-T transcripts of approximately 2.0 and 6.0 kb in the bovine tissues. Southern blot analysis indicates that the two GABA-T transcripts are encoded in a greater-than 10-kb, single-copy gene. Bovine GABA-T cDNA was expressed in E. coli using the pGEX bacterial-expression vector system. The overexpressed GABA-T was enzymatically active after purification, and it had very similar kinetic parameters when compared with those of other mammalian GABA-Ts.",

keywords = "GABA-T, Northern Blot, Southern Hybridization, cDNA Library",

author = "Jeon, \{Seong Gyu\} and Bahn, \{Jae Hoon\} and Jang, \{Joong Sik\} and Jang, \{Sang Ho\} and Lee, \{Byung Ryong\} and Lee, \{Kil Soo\} and Jinseu Park and Kang, \{Tae Cheon\} and Won, \{Moo Ho\} and Kim, \{Hyong Bai\} and Kwon, \{Oh Shin\} and Cho, \{Sung Woo\} and Choi, \{Soo Young\}",

year = "2001",

month = aug,

day = "31",

doi = "10.1016/s1016-8478(23)17066-x",

language = "English",

volume = "12",

pages = "91--96",

journal = "Molecules and Cells",

issn = "1016-8478",

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TY - JOUR

T1 - Molecular cloning and functional expression of bovine brain GABA transaminase

AU - Jeon, Seong Gyu

AU - Bahn, Jae Hoon

AU - Jang, Joong Sik

AU - Jang, Sang Ho

AU - Lee, Byung Ryong

AU - Lee, Kil Soo

AU - Park, Jinseu

AU - Kang, Tae Cheon

AU - Won, Moo Ho

AU - Kim, Hyong Bai

AU - Kwon, Oh Shin

AU - Cho, Sung Woo

AU - Choi, Soo Young

PY - 2001/8/31

Y1 - 2001/8/31

N2 - We isolated a cDNA that encodes the bovine brain γ-aminobutyrate transaminase (GABA-T; EC 2.6.1.19) from the λgt 11 cDNA library, which showed a high degree of sequence similarity to the corresponding enzymes from various sources. Northern blot analysis revealed two differentially expressed GABA-T transcripts of approximately 2.0 and 6.0 kb in the bovine tissues. Southern blot analysis indicates that the two GABA-T transcripts are encoded in a greater-than 10-kb, single-copy gene. Bovine GABA-T cDNA was expressed in E. coli using the pGEX bacterial-expression vector system. The overexpressed GABA-T was enzymatically active after purification, and it had very similar kinetic parameters when compared with those of other mammalian GABA-Ts.

AB - We isolated a cDNA that encodes the bovine brain γ-aminobutyrate transaminase (GABA-T; EC 2.6.1.19) from the λgt 11 cDNA library, which showed a high degree of sequence similarity to the corresponding enzymes from various sources. Northern blot analysis revealed two differentially expressed GABA-T transcripts of approximately 2.0 and 6.0 kb in the bovine tissues. Southern blot analysis indicates that the two GABA-T transcripts are encoded in a greater-than 10-kb, single-copy gene. Bovine GABA-T cDNA was expressed in E. coli using the pGEX bacterial-expression vector system. The overexpressed GABA-T was enzymatically active after purification, and it had very similar kinetic parameters when compared with those of other mammalian GABA-Ts.

KW - GABA-T

KW - Northern Blot

KW - Southern Hybridization

KW - cDNA Library

UR - https://www.scopus.com/pages/publications/0035980038

U2 - 10.1016/s1016-8478(23)17066-x

DO - 10.1016/s1016-8478(23)17066-x

M3 - Article

C2 - 11561735

AN - SCOPUS:0035980038

SN - 1016-8478

VL - 12

SP - 91

EP - 96

JO - Molecules and Cells

JF - Molecules and Cells

IS - 1

ER -

Molecular cloning and functional expression of bovine brain GABA transaminase

Abstract

Keywords

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