TY - JOUR
T1 - Molecular cytogenetic analysis and phylogenetic relationship of 5S and 45S ribosomal DNA in sinomartagon Lilium species by fluorescence in situ hybridization (FISH)
AU - Lee, Hyung I.I.
AU - Younis, Adnan
AU - Hwang, Yoon Jung
AU - Kang, Yun Im
AU - Lim, Ki Byung
N1 - Publisher Copyright:
© 2014, Korean Society for Horticultural Science and Springer-Verlag GmbH.
PY - 2014/12
Y1 - 2014/12
N2 - This study was conducted to elucidate the evolution of chromosomal diversification in eight species of Lilium by fluorescence in situ hybridization (FISH) technique using ribosomal DNA probes for the 5S rDNA and 45S rDNA. A phylogenic tree was reconstructed based on the results of the sequence analysis of the non-transcribed spacer (NTS) and inter-transcribed spacer (ITS) regions. The FISH analysis showed Lilium lancifolium (2x) were composed of 3 pairs of 45S rDNA genes on short arms of chromosome # 1, 2, and 7, and 3 pairs on long arms of chromosome # 4, 6, and 11. L. callosum and L. concolor showed the same pattern with L. lancifolium. However, L. dauricum showed 3 pairs of signals on the short arm of chromosome # 1, 2, and 7, and a pair of 45S rDNA signals was detected on the long arms of chromosome # 6 by FISH. The sequence length of the ITS1 and ITS2 regions were 229 bp and 233 bp, respectively. The length of the NTS region, including 5S rDNA segment, was within the range of 474 to 678 bp. According to the phylogenic analysis, L. concolor, L. amabile and L. callosum were closely related, whereas L. lancifolium, diploid and triploid were distantly related.
AB - This study was conducted to elucidate the evolution of chromosomal diversification in eight species of Lilium by fluorescence in situ hybridization (FISH) technique using ribosomal DNA probes for the 5S rDNA and 45S rDNA. A phylogenic tree was reconstructed based on the results of the sequence analysis of the non-transcribed spacer (NTS) and inter-transcribed spacer (ITS) regions. The FISH analysis showed Lilium lancifolium (2x) were composed of 3 pairs of 45S rDNA genes on short arms of chromosome # 1, 2, and 7, and 3 pairs on long arms of chromosome # 4, 6, and 11. L. callosum and L. concolor showed the same pattern with L. lancifolium. However, L. dauricum showed 3 pairs of signals on the short arm of chromosome # 1, 2, and 7, and a pair of 45S rDNA signals was detected on the long arms of chromosome # 6 by FISH. The sequence length of the ITS1 and ITS2 regions were 229 bp and 233 bp, respectively. The length of the NTS region, including 5S rDNA segment, was within the range of 474 to 678 bp. According to the phylogenic analysis, L. concolor, L. amabile and L. callosum were closely related, whereas L. lancifolium, diploid and triploid were distantly related.
KW - GC contents
KW - cloning
KW - genetic analysis
KW - karyotype
KW - sequence analysis
UR - http://www.scopus.com/inward/record.url?scp=84921038585&partnerID=8YFLogxK
U2 - 10.1007/s13580-014-0066-x
DO - 10.1007/s13580-014-0066-x
M3 - Article
AN - SCOPUS:84921038585
SN - 2211-3452
VL - 55
SP - 514
EP - 523
JO - Horticulture Environment and Biotechnology
JF - Horticulture Environment and Biotechnology
IS - 6
ER -