Molecular evidence of recombination on Korean isolates of tomato yellow leaf curl virus by nucleotide transversions and transitions

Hyejung Lee, Jungan Park, Chung Kyoon Auh, Kyeong Yeoll Lee, Chang Seok Kim, Gwan Seok Lee, Hyuncheol Soh, Hong Soo Choi, Sukchan Lee

Research output: Contribution to journalComment/debate

2 Scopus citations

Abstract

Tomato yellow leaf curl virus (TYLCV), a member of genus Begomovirus, was isolated in Korea in 2008. We sequenced and analyzed the DNA-A of 51 TYLCV isolates from Korea, and 13 of the TYLCV isolates were selected as type representatives of TYLCV from six Korean provinces. The 13 TYLCV isolates were classified into Korea Group 1 (KG1, nine isolates) and Korea Group 2 (KG2, four isolates) based on the results of phylogenetic analysis and genome size (2774 and 2781 nucleotides, respectively). A recombination detection program 3 (RDP3) revealed two recombinations between the TYLCV Korea isolates and other TYLCV isolates [Thailand (AF206674), Iran (AJ132711), and Israel (X76319)]. TYLCV Jeju isolate was characterized by two recombination events (E1 and E2) caused by the presence of E1 in ORF V1 and C3, which may seem to be the mutations of the high nucleotide transversion and transition rate. Collectively, our results suggest that the occurrence of nucleotide transversions and transitions in TYLCV DNA-A might have induced novel recombination events within the TYLCV Korea isolates.

Original languageEnglish
Pages (from-to)378-384
Number of pages7
JournalPlant Pathology Journal
Volume27
Issue number4
DOIs
StatePublished - 2011

Keywords

  • Molecular phylogeny
  • Mutation
  • Nucleotide transversions
  • RDP3
  • Recombination
  • TYLCV

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