Molecular identification and phylogenetic characterisation of Thelohanellus kitauei - Short communication

Sang Shin; Ji Kim; Casiano Choresca; Jee Han; Jin Jun; Se Park

doi:10.1556/AVet.2012.055

Molecular identification and phylogenetic characterisation of Thelohanellus kitauei - Short communication

Sang Shin, Ji Kim, Casiano Choresca, Jee Han, Jin Jun, Se Park

Department of Veterinary Medicine

Seoul National University

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Thelohanellus kitauei was isolated from the koi Cyprinus carpio haematopterus, and the 18S rRNA gene of T. kitauei was amplified by optimised nested-PCR. The PCR product was sequenced and compared with other 18S rRNA genes of Thelohanellus species to investigate the relationships between their host specificities and infection sites. Based on the 18S rRNA sequences, T. kitauei is most closely related to T. hovorkai (which can infect the intestine). Phylogenetic analysis revealed that T. kitauei was clustered with other Thelohanellus spp. infecting Cyprininae. The present study suggests that the infection site and the host specificity (subfamily level) are reflected in the genetic relationships among Thelohanellus species.

Original language	English
Pages (from-to)	30-35
Number of pages	6
Journal	Acta Veterinaria Hungarica
Volume	61
Issue number	1
DOIs	https://doi.org/10.1556/AVet.2012.055
State	Published - 1 Mar 2013

Keywords

18S rRNA sequencing
Host specificity
Thelohanellus kitauei
Thelohanellus spp.

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10.1556/AVet.2012.055

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abstract = "Thelohanellus kitauei was isolated from the koi Cyprinus carpio haematopterus, and the 18S rRNA gene of T. kitauei was amplified by optimised nested-PCR. The PCR product was sequenced and compared with other 18S rRNA genes of Thelohanellus species to investigate the relationships between their host specificities and infection sites. Based on the 18S rRNA sequences, T. kitauei is most closely related to T. hovorkai (which can infect the intestine). Phylogenetic analysis revealed that T. kitauei was clustered with other Thelohanellus spp. infecting Cyprininae. The present study suggests that the infection site and the host specificity (subfamily level) are reflected in the genetic relationships among Thelohanellus species.",

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AU - Shin, Sang

AU - Kim, Ji

AU - Choresca, Casiano

AU - Han, Jee

AU - Jun, Jin

AU - Park, Se

PY - 2013/3/1

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AB - Thelohanellus kitauei was isolated from the koi Cyprinus carpio haematopterus, and the 18S rRNA gene of T. kitauei was amplified by optimised nested-PCR. The PCR product was sequenced and compared with other 18S rRNA genes of Thelohanellus species to investigate the relationships between their host specificities and infection sites. Based on the 18S rRNA sequences, T. kitauei is most closely related to T. hovorkai (which can infect the intestine). Phylogenetic analysis revealed that T. kitauei was clustered with other Thelohanellus spp. infecting Cyprininae. The present study suggests that the infection site and the host specificity (subfamily level) are reflected in the genetic relationships among Thelohanellus species.

KW - 18S rRNA sequencing

KW - Host specificity

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JO - Acta Veterinaria Hungarica

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Molecular identification and phylogenetic characterisation of Thelohanellus kitauei - Short communication

Abstract

Keywords

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