Molecular tagging of the Bph1 locus for resistance to brown planthopper (Nilaparvata lugens Stål) through representational difference analysis

Dong Soo Park; Min Young Song; Soo Kwon Park; Sang Kyu Lee; Jong Hee Lee; Song Yi Song; Moo Young Eun; Tae Ryong Hahn; Jae Keun Sohn; Gihwan Yi; Min Hee Nam; Jong Seong Jeon

doi:10.1007/s00438-008-0353-2

Molecular tagging of the Bph1 locus for resistance to brown planthopper (Nilaparvata lugens Stål) through representational difference analysis

Dong Soo Park, Min Young Song, Soo Kwon Park, Sang Kyu Lee, Jong Hee Lee, Song Yi Song, Moo Young Eun, Tae Ryong Hahn, Jae Keun Sohn, Gihwan Yi, Min Hee Nam, Jong Seong Jeon

Department of Farm Management

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

During brown planthopper (BPH) feeding on rice plants, we employed a modified representational difference analysis (RDA) method to detect rare transcripts among those differentially expressed in SNBC61, a BPH resistant near-isogenic line (NIL) carrying the Bph1 resistance gene. This identified 3 RDA clones: OsBphi237, OsBphi252 and OsBphi262. DNA gel-blot analysis revealed that the loci of the RDA clones in SNBC61 corresponded to the alleles of the BPH resistant donor Samgangbyeo. Expression analysis indicated that the RDA genes were up-regulated in SNBC61 during BPH feeding. Interestingly, analysis of 64 SNBC NILs, derived from backcrosses of Samgangbyeo with a BPH susceptible Nagdongbyeo, using a cleaved amplified polymorphic sequence (CAPS) marker indicated that OsBphi252, which encodes a putative lipoxygenase (LOX), co-segregates with BPH resistance. Our results suggest that OsBphi252 is tightly linked to Bph1, and may be useful in marker-assisted selection (MAS) for resistance to BPH.

Original language	English
Pages (from-to)	163-172
Number of pages	10
Journal	Molecular Genetics and Genomics
Volume	280
Issue number	2
DOIs	https://doi.org/10.1007/s00438-008-0353-2
State	Published - Aug 2008

Keywords

Bph1
Brown planthopper
CAPS
Representational difference analysis
Rice

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Park, D. S., Song, M. Y., Park, S. K., Lee, S. K., Lee, J. H., Song, S. Y., Eun, M. Y., Hahn, T. R., Sohn, J. K., Yi, G., Nam, M. H., & Jeon, J. S. (2008). Molecular tagging of the Bph1 locus for resistance to brown planthopper (Nilaparvata lugens Stål) through representational difference analysis. Molecular Genetics and Genomics, 280(2), 163-172. https://doi.org/10.1007/s00438-008-0353-2

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title = "Molecular tagging of the Bph1 locus for resistance to brown planthopper (Nilaparvata lugens St{\aa}l) through representational difference analysis",

abstract = "During brown planthopper (BPH) feeding on rice plants, we employed a modified representational difference analysis (RDA) method to detect rare transcripts among those differentially expressed in SNBC61, a BPH resistant near-isogenic line (NIL) carrying the Bph1 resistance gene. This identified 3 RDA clones: OsBphi237, OsBphi252 and OsBphi262. DNA gel-blot analysis revealed that the loci of the RDA clones in SNBC61 corresponded to the alleles of the BPH resistant donor Samgangbyeo. Expression analysis indicated that the RDA genes were up-regulated in SNBC61 during BPH feeding. Interestingly, analysis of 64 SNBC NILs, derived from backcrosses of Samgangbyeo with a BPH susceptible Nagdongbyeo, using a cleaved amplified polymorphic sequence (CAPS) marker indicated that OsBphi252, which encodes a putative lipoxygenase (LOX), co-segregates with BPH resistance. Our results suggest that OsBphi252 is tightly linked to Bph1, and may be useful in marker-assisted selection (MAS) for resistance to BPH.",

keywords = "Bph1, Brown planthopper, CAPS, Representational difference analysis, Rice",

author = "Park, {Dong Soo} and Song, {Min Young} and Park, {Soo Kwon} and Lee, {Sang Kyu} and Lee, {Jong Hee} and Song, {Song Yi} and Eun, {Moo Young} and Hahn, {Tae Ryong} and Sohn, {Jae Keun} and Gihwan Yi and Nam, {Min Hee} and Jeon, {Jong Seong}",

year = "2008",

month = aug,

doi = "10.1007/s00438-008-0353-2",

language = "English",

volume = "280",

pages = "163--172",

journal = "Molecular Genetics and Genomics",

issn = "1617-4615",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "2",

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Park, DS, Song, MY, Park, SK, Lee, SK, Lee, JH, Song, SY, Eun, MY, Hahn, TR, Sohn, JK, Yi, G, Nam, MH & Jeon, JS 2008, 'Molecular tagging of the Bph1 locus for resistance to brown planthopper (Nilaparvata lugens Stål) through representational difference analysis', Molecular Genetics and Genomics, vol. 280, no. 2, pp. 163-172. https://doi.org/10.1007/s00438-008-0353-2

TY - JOUR

T1 - Molecular tagging of the Bph1 locus for resistance to brown planthopper (Nilaparvata lugens Stål) through representational difference analysis

AU - Park, Dong Soo

AU - Song, Min Young

AU - Park, Soo Kwon

AU - Lee, Sang Kyu

AU - Lee, Jong Hee

AU - Song, Song Yi

AU - Eun, Moo Young

AU - Hahn, Tae Ryong

AU - Sohn, Jae Keun

AU - Yi, Gihwan

AU - Nam, Min Hee

AU - Jeon, Jong Seong

PY - 2008/8

Y1 - 2008/8

N2 - During brown planthopper (BPH) feeding on rice plants, we employed a modified representational difference analysis (RDA) method to detect rare transcripts among those differentially expressed in SNBC61, a BPH resistant near-isogenic line (NIL) carrying the Bph1 resistance gene. This identified 3 RDA clones: OsBphi237, OsBphi252 and OsBphi262. DNA gel-blot analysis revealed that the loci of the RDA clones in SNBC61 corresponded to the alleles of the BPH resistant donor Samgangbyeo. Expression analysis indicated that the RDA genes were up-regulated in SNBC61 during BPH feeding. Interestingly, analysis of 64 SNBC NILs, derived from backcrosses of Samgangbyeo with a BPH susceptible Nagdongbyeo, using a cleaved amplified polymorphic sequence (CAPS) marker indicated that OsBphi252, which encodes a putative lipoxygenase (LOX), co-segregates with BPH resistance. Our results suggest that OsBphi252 is tightly linked to Bph1, and may be useful in marker-assisted selection (MAS) for resistance to BPH.

AB - During brown planthopper (BPH) feeding on rice plants, we employed a modified representational difference analysis (RDA) method to detect rare transcripts among those differentially expressed in SNBC61, a BPH resistant near-isogenic line (NIL) carrying the Bph1 resistance gene. This identified 3 RDA clones: OsBphi237, OsBphi252 and OsBphi262. DNA gel-blot analysis revealed that the loci of the RDA clones in SNBC61 corresponded to the alleles of the BPH resistant donor Samgangbyeo. Expression analysis indicated that the RDA genes were up-regulated in SNBC61 during BPH feeding. Interestingly, analysis of 64 SNBC NILs, derived from backcrosses of Samgangbyeo with a BPH susceptible Nagdongbyeo, using a cleaved amplified polymorphic sequence (CAPS) marker indicated that OsBphi252, which encodes a putative lipoxygenase (LOX), co-segregates with BPH resistance. Our results suggest that OsBphi252 is tightly linked to Bph1, and may be useful in marker-assisted selection (MAS) for resistance to BPH.

KW - Bph1

KW - Brown planthopper

KW - CAPS

KW - Representational difference analysis

KW - Rice

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U2 - 10.1007/s00438-008-0353-2

DO - 10.1007/s00438-008-0353-2

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C2 - 18553105

AN - SCOPUS:48349134185

SN - 1617-4615

VL - 280

SP - 163

EP - 172

JO - Molecular Genetics and Genomics

JF - Molecular Genetics and Genomics

IS - 2

ER -

Molecular tagging of the Bph1 locus for resistance to brown planthopper (Nilaparvata lugens Stål) through representational difference analysis

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Keywords

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